膜杰作
Star Staining
分子别名（Synonym）
Spike,S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2
表达区间及表达系统（Source）
SARS-CoV-2 S2 protein, His Tag (S2N-C52H2) is expressed from human 293 cells (HEK293).
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM PB, 300 mM NaCl, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
SARS-CoV-2 S2 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H2) at 0.2 μg/mL (100 ug/well) can bind Anti-SARS-CoV-2 Spike S2 protein Antibody, Human IgG4 (AS86) (Cat. No. S2N-S86) with a linear range of 1.5-2.5 ng/mL (QC tested).
Protocol
背景（Background）
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
