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Biotinylated Human DPPIV / CD26 Protein, His,Avitag™ (active enzyme, MALS verified)

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分子别名(Synonym)

DPP4,ADABP,ADCP2,CD26,DPPIV,TP103

表达区间及表达系统(Source)

Biotinylated Human DPPIV, His,Avitag (DP4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 34 - Pro 766 (Accession # NP_001926.2).

Predicted N-terminus: Asp 34

Request for sequence

蛋白结构(Molecular Characterization)

DPPIV Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 88.4 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

DPPIV SDS-PAGE

Biotinylated Human DPPIV, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

DPPIV SEC-MALS

The purity of Biotinylated Human DPPIV, His,Avitag (Cat. No. DP4-H82E3) is more than 85% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

DPPIV ELISA

Immobilized MERS S1 protein, His Tag (Cat. No. S1N-M52H5) at 2 μg/mL (100 μL/well) can bind Biotinylated Human DPPIV, His,Avitag (Cat. No. DP4-H82E3) with a linear range of 0.8-25 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >7500 pmol/min/µg (QC tested).

Protocol

 

背景(Background)

Dipeptidyl peptidase-IV (DPPIV) is also known as adenosine deaminase complexing protein 2, DPPIV or CD26 is antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. The substrates of DPPIV are proline (or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others. DPPIV also binds the enzyme adenosine deaminase specifically and with high affinity. The significance of this interaction has yet to be established.

 

前沿进展

 
 
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DPPIV靶点信息
英文全称:Dipeptidyl peptidase IV
中文全称:二肽基肽酶IV
种类:Homo sapiens
上市药物数量:34详情
临床药物数量:31详情
最高研发阶段:批准上市
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