MARCO, SCARA2, Macrophage receptor with collagenous structure, Scavenger receptor class A member 2
Mouse MARCO (421-518), His Tag (MAR-M5246) is expressed from human 293 cells (HEK293). It contains AA Gln 421 - Ser 518 (Accession # Q60754-1).
Predicted N-terminus: His
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 36.8 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse MARCO (421-518), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
MARCO is also known as Scavenger receptor class A member 2 and SCARA2. Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria. Also plays a role in binding of unopsonized particles by alveolar macrophages. Binds to the secretoglobin SCGB3A2. MARCO ligation promotes the production of inflammatory mediators by macrophages. MARCO mediated internalization of some ligands prevents their activation of cell surface TLR4 but enables their activation of intracellular TLR3. MARCO contributes to the clearance of apoptotic cells and inhaled bacteria, mast cell mediated silicosis, and the amelioration of allergen or ozone induced lung inflammation. It is required for the organization of the splenic marginal zone and the interaction of splenic macrophages and B cells.