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Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike S1)

COA
For research use only.

IDComponentsSize
RAS051-C01Pre-coated SARS-CoV-2 Spike S1 Microplate1 plate
RAS051-C02Positive Control100 μL
RAS051-C03Negative Control100 μL
RAS051-C04HRP-Anti-Human IgM100 μL
RAS051-C0510xWashing Buffer50 mL
RAS051-C06Dilution Buffer50 mL
RAS051-C07Substrate Solution12 mL
RAS051-C08Stop Solution7 mL

Background/背景

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.

Application/应用

This kit is developed for titer measurement of Anti-SARS-CoV-2 Antibody IgM (Spike S1) in human serum.

It is for research use only.

Storage/存储

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Assay Principles/原理

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgM in serum by SARS-CoV-2 Spike S1. The kit consists of Pre-coated SARS-CoV-2 Spike S1 Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgM secondary antibody and related buffer.

Your experiment will include 4 simple steps: a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

b) Add a diluted Secondary antibody HRP-Anti-Human IgM to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

Typical Data/典型数据

Biotinylated Human ELISA

For determination of antibody titer: Determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

Protocol

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