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Mouse Anti-SARS-CoV-2 (BA.2) Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.

IDComponentsSize
RAS089-C01Pre-coated SARS-CoV-2 Spike Trimer (BA.2) Microplate1 plate
RAS089-C02SARS-CoV-2 Antibody Positive Control100 μL
RAS089-C03SARS-CoV-2 Antibody Negative Control100 μL
RAS089-C04HRP-Goat anti-Mouse IgG10 μg
RAS089-C0510xWashing Buffer50 mL
RAS089-C06Dilution Buffer50 mL
RAS089-C07Substrate Solution12 mL
RAS089-C08Stop Solution7 mL

背景(Background)

A more contagious subvariant of omicron, known as BA.2, is spreading across the globe and could soon become the dominant version of Covid-19.It's now the top variant in at least 18 countries and rapidly spreading, representing 35% of all new cases that have been genetically sequenced worldwide, up from 10 countries and 21% of cases the week before, according to new data from the World Health Organization. In the U.S, BA.2 currently makes up 3.8% of genetically sequenced Covid cases, according to the Centers for Disease Control and Prevention.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.

应用说明(Application)

This kit is developed for titer or semi-quantitative measurement of Anti-SARS-CoV-2 (BA.2) Antibody IgG (Spike Trimer) in mouse serum.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储 & 运输(Storage & Shipping)

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理(Assay Principles)

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike Trimer. The Kit consists of Pre-coated with SARS-CoV-2 Spike Trimer (BA.2) Microplate , an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.

Your experiment will include 4 simple steps:

a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

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Spike protein靶点信息
英文全称:Coronavirus spike glycoprotein
中文全称:冠状病毒刺突糖蛋白
种类:Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
上市药物数量:9详情
临床药物数量:68详情
最高研发阶段:批准上市
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