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 >  Protein>APP >APP-H51H7

Human APP / Abeta40 Protein, His Tag

分子别名(Synonym)

ABPP,APPI,Amyloid-beta A4 protein

表达区间及表达系统(Source)

Human Abeta40, His Tag (APP-H51H7) is expressed from E. coli cells. It contains AA Asp 672 - Val 711 (Accession # P05067-1).

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

APP Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 6.3 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

APP SDS-PAGE

Human Abeta40, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

APP ELISA

Immobilized Human Abeta40, His Tag (Cat. No. APP-H51H7) at 5 μg/mL (100 μL/well) can bind Aducanumab with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

 

背景(Background)

Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. It has three predominant splice variants: APP695, APP751, and APP770. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C99, which is subsequently cleaved by γ-secretase to release Aβ.

 

前沿进展

 
 
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APP靶点信息
英文全称:Beta amyloid A4 protein
中文全称:β-淀粉状蛋白A4
种类:Homo sapiens
上市药物数量:11详情
临床药物数量:39详情
最高研发阶段:批准上市
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