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Anti-SARS-CoV-2 Antibody IgG Quantitative and Titer Detection Kit (Spike S1)

For research use only.

IDComponentsSize
RAS095-C01Pre-coated SARS-CoV-2 Spike S1 Microplate1 plate
RAS095-C02Positive Control100 μL
RAS095-C03Negative Control100 μL
RAS095-C04Calibrator10.5 mL
RAS095-C05Calibrator20.5 mL
RAS095-C06Calibrator30.5 mL
RAS095-C07Calibrator40.5 mL
RAS095-C08Calibrator50.5 mL
RAS095-C09Calibrator60.5 mL
RAS095-C10HRP-Anti-Human IgG200 μL
RAS095-C1110xWashing Buffer50 mL
RAS095-C12Dilution Buffer50 mL
RAS095-C13Substrate Solution12 mL
RAS095-C14Stop Solution7 mL

Background/背景

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.

Application/应用

This kit is developed for quantitative or titer detection of Anti-SARS-CoV-2 Antibody IgG (Spike S1) in human serum. It is intended for research use only (RUO).

It is for research use only.

Reconstitution/重构方法

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Assay Principles/原理

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike protein S1. The kit consists of High-bind Plate, Spike protein S1, an Positive Control, Negative Control, Calibrator, an HRP-Anti-Human IgG secondary antibody and Dilution buffer.

Your experiment will include 4 simple steps:

a) Add your sample and Calibrator to the plate. The samples are diluted by Dilution Buffer.

b) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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