登录 | 注册    微信公众号  
搜索
 >  生物标记素蛋白>CD133 >CD3-H82Q8

Biotinylated Human CD133 Protein, His,Avitag™

热销产品推荐: SP-11 链霉亲和素包被96孔板。
利用生物素-链霉亲和素系统的高特异性和高灵敏度特性,您可将生物素化蛋白固定在链霉亲和素包被96孔板上以应用于各类实验,与非生物素体系相比检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

CD133,PROM1,PROML1,Prominin-1,AC133

表达区间及表达系统(Source)

Biotinylated Human CD133 Protein, His,Avitag (CD3-H82Q8) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - His 865 (Accession # O43490-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 20 - His 865) O43490-1

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 98.9 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>80% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

This product is not suitable for cell based experiments due to cytotoxicity of DDM.

DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.

If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 12 months under sterile conditions.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
 

电泳(SDS-PAGE)

Biotinylated Human CD133 Protein, His,Avitag (Cat. No. CD3-H82Q8) SDS-PAGE gel

Biotinylated Human CD133 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human CD133 Protein, His,Avitag (Cat. No. CD3-H82Q8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD133 (133-3) Antibody, Human IgG1 with a linear range of 0.2-16 ng/mL (QC tested).

Protocol

背景(Background)

Prominin-1 is also known as CD133, Antigen AC133, PROM1, PROML1 and MSTP061. Is used as marker for hematopoietic stem and progenitor cells (HSPC) for somatic stem cell isolation. May play a role in cell differentiation, proliferation and apoptosis . Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

 

前沿进展

 
 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
CD133靶点信息
英文全称:CD133
中文全称:CD133抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定