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 >  经MALS验证的蛋白>DLL1 >DL1-H82E5

Biotinylated Human DLL1 / Delta1 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

DLL1,Delta1,H-Delta-1

表达区间及表达系统(Source)

Biotinylated Human DLL1, His,Avitag (DL1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 540 (Accession # O00548-1).

Predicted N-terminus: Gln 18

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 18 - Gly 540) O00548-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 60.1 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human DLL1, His,Avitag (Cat. No. DL1-H82E5) SDS-PAGE gel

Biotinylated Human DLL1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human DLL1, His,Avitag (Cat. No. DL1-H82E5) MALS images

The purity of Biotinylated Human DLL1, His,Avitag (Cat. No. DL1-H82E5) is more than 90% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human DLL1, His,Avitag (Cat. No. DL1-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) with a linear range of 0.078-1.25 μg/mL (QC tested).

Protocol

背景(Background)

Delta-like protein 1 (DLL1) is also known as Drosophila Delta homolog 1 (Delta1 or H-Delta-1), which contains one DSL domain and eight EGF-like domains. DLL1 is ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. As for expression, DLL1 is expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. Furthermore, DLL1 acts as a ligand for Notch receptors. Also, DLL1 can block the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

 

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