膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-PTX Antibody, Mouse IgG2a is a Mouse monoclonal antibody recombinantly expressed from CHO cells.
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG2a | Mouse Kappa
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Chemical
免疫原（Immunogen）
PTX.
特异性（Specificity）
This product is a specific antibody specifically reacts with PTX.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-20 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-PTX Antibody, Mouse IgG2a on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-PTX Antibody, Mouse IgG2a (Cat. No. PTX-S343) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized ADC-PTX at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PTX Antibody, Mouse IgG2a (Cat. No. PTX-S343) with a linear range of 0.106-6.767 ng/mL (QC tested).
Protocol
Immobilized Human TROP-2 / TACSTD2 Protein, His Tag (MALS verified) (Cat. No. TR2-H5223) at 2 μg/mL, add increasing concentrations of ADC-PTX, and then add Monoclonal Anti-PTX Antibody, Mouse IgG2a (Cat. No. PTX-S343) at 0.54 μg/mL. Detection was performed using HRP-conjugated Goat Anti-Mouse IgG with sensitivity of 0.04 ng/mL (Routinely tested).
Protocol
背景（Background）
PTX3 is a prototypic long pentraxin consisting of a C-terminal 203-amino acid pentraxin-like domain coupled with an N-terminal 178-amino acid unrelated portion. Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. PTX3 can binds to C1q, but not C1s.
