膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8) (CD6-M782G0) is recombinantly produced from human 293 cells (HEK293).
亚型（Isotype）
Mouse IgG1/kappa
偶联（Conjugate）
Unconjugated
特异性（Specificity）
This product is a specific antibody specifically reacts with CD16/Fc gamma RIII.
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
蛋白A残留（Protein A）
<5 ppm of protein tested by ELISA.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/μg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
无菌（Sterility）
Negative
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
For long term storage, the product should be stored at liquid state at -70°C.
This product is stable after storage at:
- 2-8°C for 3 months under sterile condition;
- -70°C for 24 months.
电泳（SDS-PAGE）
Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8) (Cat. No. CD6-M782G0) is more than 95% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-Bioactivity CELL BASE
Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone 3G8) (Cat. No. CD6-M782G0) stimulates Human CD16a (158V) (Luc) Jurkat Reporter Cell. The EC50 is 0.071 μg/mL (Routinely tested).
Protocol
背景（Background）
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
