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生物工艺号
DOI: 10.1126/science.aaz9356
CD20与利妥昔单抗（RTX）关键分子的相互作用
VLP (Virus like particles) 技术平台将构象完整的膜蛋白直接聚集在细胞表面，使这些复杂的膜蛋白转变为可溶性的、高浓度的蛋白用于抗体免疫和筛选，所制备的包膜VLPs在其固有的细胞膜上显示正确折叠的多次跨膜蛋白，从而能够诱导和筛选识别靶标天然构象的功能性抗体。
多次跨膜蛋白因其跨膜域的高疏水性，在常规缓冲液中难以维持天然构象。为此，我们基于昆虫细胞及哺乳动物细胞表达系统，建立了专业的去垢剂技术平台。通过筛选，已优选出包括Detergent在内的多种高效去垢剂，可显著提升靶蛋白水溶性，确保其在溶液中形成正确的构像。
Nanodisc（纳米磷脂盘）是一种由膜支架蛋白（MSPs）与磷脂分子组装而成的磷脂双分子层结构。该结构能够在模拟天然膜环境的同时，将膜蛋白整合其中，并保持其生物学活性和良好的水溶性，从而显著扩展其应用范围。我们已获得该技术的官方专利授权，确保您的研发项目在安全、合规的技术路径上稳健推进。
“Nanodisc-pro”技术平台采用创新的骨架原位组装策略，直接在跨膜蛋白的天然膜环境附近，利用骨架环绕包裹目标蛋白及其紧密相邻的天然磷脂，形成稳定的磷脂双分子层结构。该技术免除了传统方法中破坏性的去垢剂剥离与重组步骤，最大程度保留了跨膜蛋白的天然构象与活性。
2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 3 μg/mL of Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E3) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 3 μg/mL of Human CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc) (HEK293)(Cat. No. CD0-H52H1) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (QC tested).
Protocol
Immobilized Rituximab biosimilar at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E3) with a linear range of 2-62.5 ng/mL (QC tested).
Protocol
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag (Cat. No. CD0-H52H1) with a linear range of 1-63 ng/mL (QC tested).
Protocol
Immobilized Ofatumumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E5) with a linear range of 4-63 ng/mL (in presence of DDM and CHS) (QC tested).
Protocol
Immobilized Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) at 5 μg/mL (100 μL/well) can bind Biosimilar of Obinutuzumab with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
Biotinylated Human CD20, His,Avitag (HEK293) (Cat. No. CD0-H82E5) captured on Biotin CAP-Series S Sensor Chip can bind Rituximab with an affinity constant of 1.73 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200).
Protocol
Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind MabThera® (Rituximab) with an affinity constant of 6.21 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K).
Protocol
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