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官方服务号
招聘公众号
生物工艺号
蛋白高纯度及天然结构经MALS/HPLC验证
严格的QC质检保证高批间一致性
蛋白高生物活性经ELISA/SPR/FACS等验证
Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 98%. The purity of Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) is more than 90% and the molecular weight of this protein is around 82-100 kDa verified by SEC-MALS.
Report
Immobilized Human B7-2, His Tag (Cat. No. CD6-H5223) at 10 μg/mL (100 μL/well) can bind Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Mouse CD28, His,Avitag (Cat. No. CD8-M82E3) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag (Cat. No. B71-H52A4) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
Immobilized Human CTLA-4, His Tag (Cat. No. CT4-H5229) at 5 μg/mL, add increasing concentrations of Cadonilimab (PD-1 x CTLA-4 bispecific antibody) and then add Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) at 0.2 μg/mL. (Routinely tested).
Protocol
Biotinylated Human CTLA-4, His,Avitag (Cat. No. CT4-H82E3) captured on Biotin CAP - Series S sensor Chip can bind Yervoy (Ipilimumab) with an affinity constant of 0.635 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
![使用B7-1 [Biotinylated] : CTLA-4 抑制剂筛选试剂盒检测抗CTLA-4中和抗体对B7-1: CTLA-4结合的抑制效果](https://ehf85k.acrobiosystems.cn/public/images/CKP_CTLA4/CTLA4DataA8.jpg)
Serial dilutions of Anti-CTLA-4 Neutralizing antibody (Catalog # EP116-C04) (1:1 serial dilution, from 10 μg/mL to 0.078μg/mL) was added into CTLA-4: Biotinlated B7-1 binding reactions. The assay was performed according to the protocol described below. (QC tested).
Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay.
Immobilized Human OX40 Protein, His Tag (MALS verified) (Cat. No. OX0-H5224) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, His, Avitag (Cat. No. CT4-H82E3) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).
Protocol
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