产品详情
抗体来源(Source)
Anti-Adalimumab Antibody (AY23b) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号(Clone)
AY23b
种属(Species)
Mouse
亚型(Isotype)
Mouse IgG1 | Mouse Kappa
抗体类型(Antibody Type)
Recombinant Monoclonal
种属反应性(Reactivity)
Human
免疫原(Immunogen)
Adalimumab
特异性(Specificity)
Recognizes Adalimumab specifically.
应用(Application)
ApplicationRecommended UsageELISA0.2-100 ng/mL纯度(Purity)
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
纯化(Purification)
Protein A purified / Protein G purified
制剂(Formulation)
Lyophilized from a 0.22 μm-filtered solution in pH 7.5 containing 50 mM Tris, 100 mM Glycine, 25 mM Arginine and 150 mM NaCl, with trehalose as protectant.
Please contact us for customized product forms or formulations.
重构方法(Reconstitution)
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Anti-Adalimumab Antibody (AY23b) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) is more than 95% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) at 2 μg/mL, add increasing concentrations of Adaliumumab and then add Biotinylated Biotinylated Anti-Adalimumab Antibody (AY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (QC tested).
Protocol
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