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抗体来源（Source）
Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) is a neutralizing rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.
抗体类型（Antibody Type）
Recombinant Monoclonal
克隆号（Clone）
1G10
亚型（Isotype）
Rabbit IgG, Kappa
宿主（Host Species）
Rabbit
免疫原（Immunogen）
Recombinant FMC63 scFv derived from HEK293 cells
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
特异性（Specificity）
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
应用（Application）
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).
推荐稀释比（Recommended Dilution）
1:50
产品形式（Form）
Lyophilized
制剂（Formulation）
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
重构方法（Reconstitution）
Please refer to the Certificate of Analysis (CoA) for specific instructions.
运输（Shipping）
Lyophilized product is shipped at ambient temperature.
存储（Storage）
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8°C for 12 months after reconstitution.
合规声明（Notices）
Product Specific Notices: For research use only.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG1 (1G10) (Cat. No. FM3-PFM721) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG1 (1G10) (Cat. No. FM3-PFM721) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG1 (1G10) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
活性（Bioactivity）-FACS Analysis of Blood Samples
After Fc receptor blockade, whole blood only control (Figure A) and whole blood spiked with CAR-T cells (Figure B) were first stained with PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (Cat. FM3-PFM721), following 7-AAD staining. The data were analyzed using FCS Express 7 software (Routinely tested). CAR-T cells were identified as PE-positive events within the live CD45+CD3+ (or CD45+) lymphocyte population. The proportion of CD45+CAR+ cells is around 20%, which is largely consistent with the theoretical spike-in value.
Protocol
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Non-specific binding to Human whole blood
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (0.03% Proclin) (Cat. No. FM3-PFM721) binding to CD3+ cells present in human whole blood. 100 μl of human whole blood were simultaneously stained with APC/Cyanine7 anti-human CD3 Antibody and PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (2 μL of the antibody stock solution in a final volume of 100 µL), compared with isotype control antibody and positive control antibody. Both APC/Cyanine7 and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells.
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