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抗体来源（Source）
Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 is a mouse monoclonal antibody recombinantly expressed in HEK293 cells.
抗体类型（Antibody Type）
Recombinant Monoclonal
亚型（Isotype）
Mouse IgG1, Kappa
免疫原（Immunogen）
MMAE
特异性（Specificity）
Specifically recognizes the target-MMAE&MMAF.
纯化（Purification）
Protein A purified / Protein G purified.
浓度（Concentration）
Please refer to the Certificate of Analysis (CoA).
产品形式（Form）
Lyophilized
制剂（Formulation）
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
重构方法（Reconstitution）
Please refer to the Certificate of Analysis (CoA) for specific instructions.
运输（Shipping）
Lyophilized product is shipped at ambient temperature.
存储（Storage）
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
合规声明（Notices）
Product Specific Notices: For research use only.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-ELISA
Immobilized Mouse Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-M5252) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 50% Mouse serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized ADC-MMAF at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-M5252) with a linear range of 0.5-1.56 ng/mL (Routinely tested).
Protocol
Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-M5252) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
交叉验证-Cross Verification
ELISA binding of Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-M5252) with Trastuzumab Deruxtecan, IgG1-MMAF, Sacituzumab Govitecam, Disitamab Vedotin (RC48), Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-M5252), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Trastuzumab Deruxtecan, IgG1-MMAF, Sacituzumab Govitecam, Disitamab Vedotin (RC48), Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was Peroxidase AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) (Jackson, Cat. No. 109-035-098) used at 1:12000 concentration.
Monoclonal Anti-MMAE and MMAF Antibody, Mouse IgG1 (Cat. No. MME-M5252) specifically recognizes the MMAE and MMAF payloads. In this assay, it shows binding to ADCs carrying MMAE or MMAF (Disitamab Vedotin (RC48), Trastuzumab MMAE, and a model IgG1-MMAF conjugate), and shows no cross-reactivity with ADCs carrying different payloads (Trastuzumab Deruxtecan (DXd), Sacituzumab Govitecan (SN-38), and Trastuzumab-DM1) under the tested conditions (Routinely tested).
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