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组分（Materials Provided）
IDComponentsSizeOPA-R006-012×qPCR Master Mix1.0 mL×2OPA-R006-02HEK293 Primer & Probe Mix700 μLOPA-R006-03HEK293 DNA Control（3ng/μL）100 μLOPA-R006-04Dilution Buffer1.5 mL×3OPA-R006-05DNase/RNase-Free Water1.0 mL产品概述（Product Overview）
HEK293 resDNA Quantitation Kit is designed for quantitative detection of residual HEK293 DNA in biopharmaceutical productions (Antibodies, cells, viruses, etc.). Based on real-time quantitative PCR (qPCR) method, this kit makes detection the residual DNA form HEK293 cells rapid and reliable. The lower limit of quantitation is 30 fg/µL. All procedures are typically in less than 4 hours.
Use the kit after you extract host cell DNA from test samples. For achieving the better DNA recovery, it is recommended to use the resDNA Sample Preparation Kit Ⅱ (Magnetic Beads) (Cat. No. OPA-R024) in combination.
应用说明（Application）
The kit is used for quantitative detection of residual HEK293 DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
产品特性（Features）
- High sensitivity for optimal product safety: LLOD (15 fg/µL) for detection of residue host cell DNA
- High specificity: No cross-reactivity with unrelated DNA
- Validation: ICH Q2(R2) as validation of analytical procedures
- High-quality: This kit is manufactured in ISO 13485 standard facility.
存储（Storage）
Unopened kit should be stored at -15℃ to -25℃ upon receiving.
质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
Figure 1. High sensitivity and broad dynamic range using the HEK293 resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 5 (30 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of HEK293 genomic DNA (included in the kit) in the presence of CHO DNA and E. coli DNA.
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