关注公众号
收藏产品 产品详情
组分（Materials Provided）
IDComponentsSizeOPA-R017-012×qPCR Master Mix1.0 mL×2OPA-R017-02Pichia Primer & Probe Mix700 μLOPA-R017-03Pichia DNA Control（3ng/μL）100 μLOPA-R017-04Dilution Buffer1.5 mL×3OPA-R017-05DNase/RNase-Free Water1.0 mL产品概述（Product Overview）
Pichia pastoris resDNA Quantitation Kit is designed for quantitative detection of residual Pichia pastoris DNA in biopharmaceuticals (Antibodies, Vaccines, etc.). Based on real-time quantitative PCR (qPCR) method, this kit makes the detection of the residual DNA from Pichia pastoris rapid and reliable. The lower limit of quantitation is 3 fg/µL. All procedures are typically in less than 4 hours.
Use the kit after you extract host cell DNA from test samples. To achieve the best performances, resDNA Sample Preparation Kit Ⅱ(Magnetic Beads) (Cat. No. OPA-R024) is recommended for DNA extraction.
应用说明（Application）
The kit is used for quantitative detection of residual Pichia pastoris DNA in biopharmaceuticals.
For use in quality control/manufacturing process only.
It is for research use only.
产品特性（Features）
- High sensitivity for optimal product safety: LLOD (1 fg/µL) for detection of residue host cell DNA
- High specificity: No cross-reactivity with unrelated DNA
- Validation: ICH Q2(R2) as Validation of Analytical Procedures
- High-quality: This kit is manufactured in ISO 13485 standard facility.
存储（Storage）
Unopened kit should be stored at -15℃ to -25℃ upon receiving.
质量管理控制体系（QMS）
推荐产品
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
Figure 1. High sensitivity and broad dynamic range using the Pichia pastoris resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 6 (3 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of Pichia pastoris genomic DNA (included in the kit) in the presence of DNA (CHO, E. coli and HEK293).
用户评价 发表评论
联系我们 
项目合作 