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分子别名（Synonym）
Glycoprotein/G Protein (RABV)
表达区间及表达系统（Source）
Rabies virus (strain CVS-11) Glycoprotein G, His Tag (RAG-V55H5) is expressed from Baculovirus-Insect cells. It contains AA Lys 20 - Lys 458 (Accession # ADJ29911.1).
Predicted N-terminus: Lys 20
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 53.6 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Within the expressed region, several mutations were introduced to optimize the protein expression and stability.
The protein is designed as a trimer.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rabies virus (strain CVS-11) Glycoprotein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rabies virus (strain CVS-11) Glycoprotein G, His Tag (Cat. No. RAG-V55H5) is more than 85% and the molecular weight of this protein is around 165-230 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rabies virus (strain CVS-11) Glycoprotein G, His Tag (Cat. No. RAG-V55H5) at 1 μg/mL (100 μL/well) can bind Mouse Glycoprotein G Antibody, Mouse IgG1 (523-11) (Cat. No. RAG-M305) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (Cat. No. GLN-M645) captured on Protein A Chip can bind Rabies virus Glycoprotein G, His Tag (Cat. No. RAG-V55H5) with an affinity constant of 1.25 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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