抗体特性经Flow Cytometry验证并免费提供Protocol
| Molecule | Cat. No. | Species | Source | Product Description |
|---|---|---|---|---|
| FMC63 | FM3-Y45 | Mouse | Hybridoma | Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) |
| FM3-Y45P1 | Mouse | HEK293 | Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (HEK293) | |
| FM3-FY45 | Mouse | Hybridoma | FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) New | |
| FM3-BY45 | Mouse | Hybridoma | Biotinylated Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) New | |
| FM3-BY54 | Mouse | HEK293 | Biotinylated Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1, Avitag™ (Y45) | |
| FM3-HPY53 | Mouse | HEK293 | PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) |
2e5 of FMC63 scFv-based anti-CD19 CAR-293 cells were stained with 100 µL of the working solution of Monoclonal AntiFMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) and isotype control respectively, washed and then followed by PE anti-mouse IgG1 Antibody and analyzed with flow cytometry (QC tested).
Protocol
Non-specific binding of Monoclonal Anti-FMC63 scFv Antibody (Cat. No. FM3-Y45) to non-transfected 293 cells was determined by flow cytometry. The data showed that Anti-FMC63 scFv Antibody didn’t bind to non-transfected 293 cells.
Protocol
Non-specific binding of Monoclonal Anti-FMC63 scFv Antibody (Cat. No. FM3-Y45) to non-transfected human PBMCs was determined by flow cytometry. The data showed that Anti-FMC63 scFv Antibody didn’t bind to non-transfected human PBMCs.
Protocol
Immobilized FMC63 scFv, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Clone Y45) with a linear range of 1-19 ng/mL. Anti-DNP antibody, mouse IgG1 (Cat. No. DNP-M1) was used as an isotype control (QC tested).
Protocol
ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag was inhibited by increasing concentration of Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Clone Y45). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).
Protocol
Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay
Protocol
| Molecule | Cat. No. | Product Description |
|---|---|---|
| CD19 | CD9-HP2H3 | PE-Labeled Human CD19 (20-291) Protein, His Tag New |
| CD9-HF2H2 | FITC-Labeled Human CD19 (20-291) Protein, His Tag DMF Filed | |
| CD9-HF251 | FITC-Labeled Human CD19 (20-291) Protein, Fc Tag DMF Filed | |
| CD9-H8259 | Biotinylated Human CD19, Fc Tag, ultra sensitivity (primary amine labeling) DMF Filed | |
| CD9-H82F7 | Biotinylated Human CD19 (20-291) Protein, Fc,Avitag™ New | |
| CD9-H82E9 | Biotinylated Human CD19 (20-291) Protein, His,Avitag™ (SPR verified) New | |
| MBS-K005 | CD19 protein-coupled magnetic beads | |
| CD9-H5259 | Human CD19 (20-291) Protein, Fc Tag, low endotoxin | |
| CD9-H5258 | Human CD19 (20-291) Protein, Mouse IgG2a Fc Tag | |
| CD9-H52H2 | Human CD19 (20-291) Protein, His Tag DMF Filed | |
| CD9-H5250 | Human CD19 (20-291) Protein, Llama IgG2b Fc Tag, low endotoxin | |
| CD9-H5251 | Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity) DMF Filed | |
| CD9-C5251 | Cynomolgus / Rhesus macaque CD19 (20-292) Protein, Fc Tag | |
| CD9-C5221 | Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His Tag |
