搜索
>产品 > Anti-FMC63 scFv抗体

更加灵敏检测Anti-CD19 (FMC63) CAR的特异性抗体

抗体特性经Flow Cytometry验证并免费提供Protocol

背景
CD19是目前CAR-T细胞治疗领域中应用最广泛的靶点,大量研究表明CD19是治疗B-ALL,CLL和B细胞淋巴瘤有效且安全的靶点。FMC63是一种靶向人CD19的鼠单克隆抗体。到目前为止,大多数报道的CART19试验项目均是以FMC63抗体序列为基础构建的CAR-T,包括FDA批准上市的两种Anti-CD19 CAR-T细胞药物Kymriah和Yescarta。根据2018年中检院颁布的《CAR-T细胞治疗产品质量控制检测研究及非临床研究考虑要点》,CAR转染阳性率的检测应采用流式细胞法,推荐使用具有更好专属性的针对抗原结合部位的抗原蛋白或抗独特型抗体进行检测。
然而市面上现存的CD19抗原大多灵敏度不够而无法满足检测需求,ACROBiosystems开发的高亲和力人CD19蛋白在检测Anti-CD19 CAR阳性表达率方面显现出了高特异性、高灵敏度的特性,被广泛用于Anti-CD19 CAR-T开发过程中慢病毒包装后的滴度测定、CAR基因转染/转导效率的检测和Anti-CD19 CAR-T细胞的质控放行中,获得了业内的广泛认可。
高亲和力人CD19蛋白之后,ACROBiosystems持续发力推出特异性靶向FMC63 scFv抗原识别表位的抗独特型抗体,进一步丰富了FMC63 CAR的检测手段。此抗体同高亲和力人CD19蛋白一样具有灵敏度高,特异性好的特点,经充分验证可适用于Flow Cytometry (FCM)方法检测Anti-CD19 (FMC63) CAR的表达。
产品列表
Molecule Cat. No. Species Source Product Description
FMC63 FM3-Y45 Mouse Hybridoma Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45)
FM3-FY45 Mouse Hybridoma FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) New
FM3-BY45 Mouse Hybridoma Biotinylated Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) New
产品特点
能够高特异无背景检测Anti-CD19 (FMC63) CAR-T临床样本
Detection of Anti-CD19 (FMC63) CAR Expression

2e5 of FMC63 scFv-based anti-CD19 CAR-293 cells were stained with 100 µL of the working solution of Monoclonal AntiFMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) and isotype control respectively, washed and then followed by PE anti-mouse IgG1 Antibody and analyzed with flow cytometry (QC tested).

Protocol

Assessment of Non-specific Binding to 293 Cells

Non-specific binding of Monoclonal Anti-FMC63 scFv Antibody (Cat. No. FM3-Y45) to non-transfected 293 cells was determined by flow cytometry. The data showed that Anti-FMC63 scFv Antibody didn’t bind to non-transfected 293 cells.

Protocol

Assessment of Non-specific Binding to PBMCs

Non-specific binding of Monoclonal Anti-FMC63 scFv Antibody (Cat. No. FM3-Y45) to non-transfected human PBMCs was determined by flow cytometry. The data showed that Anti-FMC63 scFv Antibody didn’t bind to non-transfected human PBMCs.

Protocol

能够更灵敏且特异地识别Anti-CD19 (FMC63) CAR的抗原结合位点
Binding Activity and Specificity Measured by ELISA

Immobilized FMC63 scFv, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) with a linear range of 1-19 ng/mL. Anti-DNP antibody, mouse IgG1 (Cat. No. DNP-M1) was used as an isotype control (QC tested).

Protocol

Neutralizing Activity Measured by ELISA

ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag was inhibited by increasing concentration of Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).

Protocol

High Affinity Determined by SPR

Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay

Protocol

相关产品
高亲和力CD19蛋白产品列表
Molecule Cat. No. Product Description
CD19 CD9-HP2H3 PE-Labeled Human CD19 (20-291) Protein, His Tag New
CD9-HF2H2 FITC-Labeled Human CD19 (20-291) Protein, His Tag DMF Filed
CD9-HF251 FITC-Labeled Human CD19 (20-291) Protein, Fc Tag DMF Filed
CD9-H8259 Biotinylated Human CD19, Fc Tag, ultra sensitivity (primary amine labeling) DMF Filed
CD9-H82F7 Biotinylated Human CD19 (20-291) Protein, Fc,Avitag™ New
CD9-H82E9 Biotinylated Human CD19 (20-291) Protein, His,Avitag™ (SPR verified) New
MBS-K005 CD19 protein-coupled magnetic beads
CD9-H5255 Human CD19 (20-278) Protein, Fc Tag
CD9-H5259 Human CD19 (20-291) Protein, Fc Tag, low endotoxin
CD9-H5258 Human CD19 (20-291) Protein, Mouse IgG2a Fc Tag
CD9-H52H2 Human CD19 (20-291) Protein, His Tag DMF Filed
CD9-H5250 Human CD19 (20-291) Protein, Llama IgG2b Fc Tag, low endotoxin
CD9-H5251 Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity) DMF Filed
CD9-C5251 Cynomolgus / Rhesus macaque CD19 (20-292) Protein, Fc Tag
更多CAR-T相关产品
> 点击查看更多CAR-T靶点信息 > 点击查看更多CD19详细信息——高亲和力CD19蛋白,专为检测CD19-CAR-T而设计 > 点击查看 anti-rituximab antibody -专用于检测Anti-CD20 (Rituximab) CAR的高特异性抗体
*如果您希望ACRO开发更多类型的Anti-FMC63 Antibody产品, 请 点击提议
*如果您希望ACRO补充更多Anti-FMC63 Antibody产品的实验设计和应用数据,请 点击咨询

消息提示

请输入您的联系方式,再点击提交!

确定