Human LAG-3, mouse IgG2a Fc tag (LA3-H52Aa) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
Predicted N-terminus: Leu 23
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 73.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human LAG-3, mouse IgG2a Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-Human LAG3 MAb, Human IgG1 at 0.5 μg/mL (100 μL/well) can bind Human LAG-3, mouse IgG2a Fc tag (Cat. No. LA3-H52Aa) with a linear range of 0.8-3 ng/mL (QC tested).
Immobilized Human LAG-3, mouse IgG2a Fc tag (Cat. No. LA3-H52Aa) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque FGL1, Fc Tag (Cat. No. FG1-C5253) with a linear range of 8-125 ng/mL (Routinely tested).
Flow Cytometry assay shows that recombinant human LAG3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa ) can bind to daudi cell surface. The concentration of LAG3 mFc used is 0.3μg/ml (Routinely tested).
FACS analysis shows that the binding Human LAG3, Mouse IgG2a Fc Tag to daudi cells was inhibited by increasing concentration of neutralizing anti-LAG3 antibody. The concentration of LAG3 mFc used is 1 μg/ml. The IC50 is 0.165 μg/ml (Routinely tested).
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.