膜杰作
Star Staining
分子别名（Synonym）
CD19,B4,CVID3,MGC12802
表达区间及表达系统（Source）
FITC-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HF2H2) is expressed from human HEK293 cells. It contains AA Pro 20 - Lys 291 (Accession # P15391-1). It is the FITC labeled form of Human CD19, His Tag (Cat. No. CD9-H52H2).
Predicted N-terminus: Pro 20
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 32.0 kDa. The protein migrates as 47-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
蛋白标记度（Protein Ratio）
The FITC to protein molar ratio is 2-4.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
FITC-Labeled Human CD19 (20-291), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-FACS
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HF2H2) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
+添加评论
- 250XXXXXXX
- FITC-Labeled Human CD19 (20-291) Protein, His Tag DMF Filed, is a valuable product for CD19 CAR staining. Reliable lots. Excellent lead time and customer service. Packaging is excellent to ensure product arrives undamaged. The vials are easy to reconstitute for reagent aliquoting. Every time I have ordered FITC-Labeled Human CD19 (20-291) Protein, the product has arrived on time with a printed certificate of analysis, with specified shipping and storage requirements.
- 2022-6-14
- 188XXXXXXX1
- 购买本抗体主要用于检测car-cd19 mRNA在目的细胞的表达情况。转染mRNA后，细胞表达car-cd19，fitc偶联的cd19抗体能够直接检测 无需二抗非常方便
- 2024-3-22
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背景（Background）
B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.
