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 >  Protein>CD47 >CD7-H5227

Human CD47 Protein, His Tag (MALS verified)

分子别名(Synonym)

CD47,MER6,IAP,OA3

表达区间及表达系统(Source)

Human CD47, His Tag (CD7-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3 ).

Predicted N-terminus: Gln 19

Request for sequence

蛋白结构(Molecular Characterization)

CD47 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.6 kDa. The protein migrates as 33-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD47 SDS-PAGE

Human CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

CD47 SEC-MALS

The purity of Human CD47, His Tag (Cat. No. CD7-H5227) is more than 90% and the molecular weight of this protein is around 24-36 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CD47 ELISA

Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 31-125 ng/mL (QC tested).

Protocol

CD47 BATCH ELISA
CD47 ELISA

Immobilized Monoclonal Anti-Human CD47 Antibody, Human IgG4 at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 0.1-0.8 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

CD47 SPR

Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human CD47, His Tag (Cat. No. CD7-H5227) with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

CD47 BLI

Loaded Anti-Human CD47 MAb (Human IgG4) on AHC Biosensor, can bind Human CD47, His Tag (Cat. No. CD7-H5227) with an affinity constant of 6.18 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

文献引用(Citations)

 

前沿进展

 
 
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CD47靶点信息
英文全称:Cluster of differentiation 47
中文全称:分化群47
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:55详情
最高研发阶段:临床三期
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