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Biotinylated Human Fc gamma RIIA / CD32a (H167), His Tag, Avi Tag (Avitag™) (SPR verified)

分子别名(Synonym)

CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2

表达区间及表达系统(Source)

Biotinylated Human CD32a (H167), His Tag, Avi Tag (SPR verified) (CDA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1).

Predicted N-terminus: Ala 36

蛋白结构(Molecular Characterization)

Online(Ala 36 - Ile 218) P12318-1

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 24.0 kDa. The protein migrates as 30-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD32a (H167), His Tag, Avi Tag (SPR verified) (Cat. No. CDA-H82E6) SDS-PAGE gel

Biotinylated Human CD32a (H167), His Tag, Avi Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human CD32a (H167), His Tag, Avi Tag (Cat. No. CDA-H82E6) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore T200) (QC tested).

点击申请Protocol

Biotinylated Human SPR

Immobilized Biotinylated Human CD32a (H167), His Tag, Avi Tag (Cat. No. CDA-H82E6) on SA Chip can bind Rituximab with an affinity constant of 0.74 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

点击申请Protocol

 

背景(Background)

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

 

专利使用说明(Limited Use License)

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

 

前沿进展

货号/价格

CDA-H82E6-25ug / ¥3500

CDA-H82E6-200ug / ¥13800

文档
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021-31604788(上海)
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order.cn@acrobiosystems.com
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techsupport@acrobiosystems.com

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