分子别名(Synonym)
PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
表达区间及表达系统(Source)
MABSol® Biotinylated Human PCSK9, Avitag,His Tag (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).
Predicted N-terminus: Gln 31
蛋白结构(Molecular Characterization)

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.9 kDa respectively. The protein migrates as 20 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Biotinylation)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Biotin:Protein Ratio)
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>92% as determined by reduced SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)

Biotinylated Human PCSK9, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
活性(Bioactivity)-ELISA

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) with a linear range of 2-31 ng/mL (QC tested).
Protocol

Report

Immobilized Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) can bind PCSK9 Mab, Human IgG with a linear range of 0.05-1.5 ng/mL. The biotinylated PCSK9 is coated at 1 μg/mL (100 μL/well) on a streptavidin coated plates (Routinely tested).
Protocol

Immobilized Human VLDL R, His Tag (Cat. No. VLR-H5227) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) with a linear range of 10-156 ng/mL (Routinely tested).
Protocol
活性(Bioactivity)-FACS

FACS analysis shows that Biotinylated Human PCSK9, Avitag,His Tag (Cat. No. PC9-H82E7) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 1.502-2.378 μg/mL.
Protocol
文献引用(Citations)
Authors: Wang CK, Amiss AS, Weidmann J
Journal: J Biol Chem 2020
Application: SPR
Oligonucleotides Comprising Modified Nucleosides
Authors: J Carter, et al
Journal: US20190177730A1 2019
Application: ELISA
Calcium-mediated allostery of the EGF fold
Authors: Wang CK, et al.
Journal: ACS Chem Biol 2018
Application: SPR
Evolution of sequence-defined highly functionalized nucleic acid polymers
Authors: Chen Z, et al.
Journal: Nat Chem 2018
Application: SPR
背景(Background)
Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).