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ActiveMax® Recombinant Human Activin A / INHBA, low endotoxin

分子别名(Synonym)

Activin A,INHBA

表达区间及表达系统(Source)

ActiveMax® Human Activin A,Tag Free, low endotoxin (ACA-H421b) is expressed from human 293 cells (HEK293). It contains AA Gly 311 - Ser 426 (Accession # AAH07858.1).

Predicted N-terminus: Gly 311

蛋白结构(Molecular Characterization)

Unconjugated(Gly 311 - Ser 426) AAH07858.1

This protein carries no "tag".

The protein has a calculated MW of 13 kDa. the protein migrates as 14 kDa under reducing (R) condition, and 27 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 0.056% TFA in 60% ACN. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) SDS-PAGE gel

ActiveMax® Human Activin A,Tag Free, low endotoxin on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) at 10μg/mL (100 μL/well) can bind Human ACVR1B, Fc Tag (Cat. No. ACB-H5251) with a linear range of 0.3-2.5 μg/mL (QC tested).

点击申请Protocol

Biotinylated Human SPR

Immobilized ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) at 2 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. ACA-H5253) with a linear range of 0.08-1.25 μg/mL (QC tested).

点击申请Protocol

Biotinylated Human ELISA

The bio-activity of ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically ≤ 9.5ng/ml (Routinely tested).

点击申请Protocol

文献引用(Citations)

背景(Background)

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely inhibin down regulates FSH synthesis and inhibits FSH secretion.Activins are nonglycosylated homodimers or heterodimers of various β subunits (βA, βB, βC, and βE in mammals), while Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a widely expressed homodimer of two βA chains. The βA subunit can also heterodimerize with a βB or βC subunit to form Activin AB and Activin AC, respectively. The 14 kDa mature human βA chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat βA.

 

前沿进展

货号/价格

ACA-H421b-25ug / ¥2500

ACA-H421b-100ug / ¥6500

ACA-H421b-1mg (200ug x 5) / ¥42500

产品定制
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-31604788(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com

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