Star Staining，闪亮登场！10月11-12日，ACROBiosystems百普赛斯携专为CAR-T细胞质控和临床样本分析检测而设计的全新产品“Star Staining”亮相第五届免疫基因及细胞治疗论坛——免疫基因及细胞治疗领域产学研医转化影响力最高的年度品牌盛会之一。高级研发经理石晓娟于细胞免疫治疗创新与研发论坛分享报告《CAR-T细胞药物开发过程中的关键试剂选择》。
荧光标记蛋白是检测CAR表达的优选试剂。荧光标记技术是开发高质量荧光标记蛋白的关键。目前行业主流的标记技术因采用传统“随机”化学标记方法，无法保证标记荧光的均一性且标记位点可能会覆盖蛋白的活性位点而影响蛋白本身活性，很难获得高批间一致性、高活性的荧光标记蛋白。ACROBiosystems百普赛斯弥补空白，以“星级”的研发实力，推出“星级”的技术平台及产品——Star Staining，专为CAR-T细胞质控和临床样本分析检测而设计。
石晓娟女士向来宾分享道，“Star Staining可将荧光分子特异性标记到蛋白的特定位点，标记速度快效率高，且不影响蛋白的天然空间构象。此系列荧光标记产品具有灵敏度高，批间差小，低背景等特点，被广泛应用于CAR-T的质控放行和临床样本的检测过程中。“Star Staining定点标记技术成为开发高质量荧光标记蛋白产品的有力武器，从而加速生物药研发进程。
ACROBiosystems百普赛斯在大会设有展位，与现场来宾进行了深入互动。
Star Staining到底优秀到哪方面呢？看了下面的数据，您就明白啦~
>>>>Star Staining FITC-labeled Human BCMA产品纯度高于95%。
>>>>Star Staining FITC-labeled Human BCMA和CD19蛋白产品的检测灵敏度显著高于竞品。
The binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA and CD19 protein have a much higher binding activity than that of the other competitor.
>>>>Star Staining系列产品经未转染CAR的PBMC细胞双染批检测验证，无非特异背景问题，特异性显著高于竞品，保证CAR表达检测结果的特异性和准确性，适用于CAR-T临床样本分析。
Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.
>>>>Star Staining FITC-labeled Human BCMA产品可保持天然蛋白活性。
1. FACS验证结果显示标记FITC前后BCMA蛋白天然活性保持不变。
The binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled and unconjugated Human BCMA, His Tag have almost the same level of binding activity.
2. SPR验证结果显示标记FITC前后BCMA蛋白的亲和力水平无差异。
The binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled and unconjugated Human BCMA, His Tag have almost the same level of affinity.
>>>> Star Staining FITC-labeled Human BCMA产品不同批次、不同规格的蛋白活性保持一致，具有高批间一致性，以确保长期用于CAR-T细胞质控和临床样本分析中检测结果的稳定性和可靠性。
Binding activity of two different sizes (left) or three different lots(right) of FITC-Labeled Human BCMA against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
>>>>高稳定性：25℃条件下加速48h和反复冻融三次，依然可保持较高的生物活性，显示出蛋白的高度稳定性，可放心储存和使用。
FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) in different concentrations at 25℃ for 48 hours and freeze-throw cycles are stable without performance reduction.
参考文献：
[1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print, 2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.
[2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.
[3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.
Star Ribbon预染蛋白Marker蛋白质标记物是生物研究和药物开发的重要组成部分。无论是用于蛋白质电泳还是western blot，我们的预染色蛋白质标记物帮助您快速确定目标蛋白质的分子量或评估转移效率。Fc受体蛋白治疗性抗体的功效取决于Fab片段及其对目标抗原的结合活性，还取决于Fc片段及其与关键Fc受体的相互作用。因此，在抗体工程中候选物必须针对一系列受体进行测试。探索我们的重组Fc受体蛋白质的全面收藏！
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