在与PD-(L)1联用过程中，有一些效果是挺不错的，有些效果却差强人意，受限很大一部分的原因在于CD40激动剂的治疗窗口太窄；另一个可能的原因是PD-1是诱导型表达，当血液中的T细胞被激活，PD-1抗体在血液中暴露量过多，减少了肿瘤中的暴露量。使其疗效大打折扣。需要更多的临床前研究来进一步确定这些药物组合的作用机制，并改进药物和方案的剂量和顺序。相信不久的将来会产生更多令人鼓舞的临床数据，提高治疗效果，并拓宽更多癌症患者的治疗选择。
ACROBiosystems从靶点蛋白开始，致力于更好的生物医药，已针对激动型抗体多种热门靶点开发了优化了一系列高纯度、不同种属、不同标签、天然聚体结构经MALS验证、高生物活性经ELISA/SPR/BLI/Cell based assay等验证的产品，适用于免疫、抗体筛选、细胞活性检测等实验。
CD40Ligand（activetrimer）产品列表
★CD40 Ligand三聚体结构经MALS验证
Thepurity of Human CD40 Ligand, Fc Tag (active trimer) (MALS verified)(Cat. No. CDL-H5269)is more than 90% in HP-SEC, and the molecular weight of this proteinis around 160-180 kDa verified by SEC-MALS.
★高生物活性经ELISA/SPR/BLI/Cell based assay验证
>>>ELISA
Immobilized Human CD40, His Tag (Cat. No. CD0-H5228) at 1 μg/mL (100 μL/well)can bind Human CD40 Ligand, Fc Tag (Cat. No. CDL-H5267) with a linearrange of 0.2-8 ng/mL (QC tested).
>>>BLI
Loaded Human CD40, Fc Tag (Cat. No. CD0-H5253) on Protein A Biosensor, canbind Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) with anaffinity constant of 1.58 nM as determined in BLI assay (ForteBioOctet Red96e) (Routinely tested).
>>>Cell based assay
HumanCD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) stimulates secretionof IL-6 by human B cells. The ED50 for this effect is 18.76-19.45ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4(Routinely tested).
Neutralization assay shows that the cytokine secretion effect of Human CD40 Ligand,His,Flag Tag (Cat. No. CDL-H52Db) was inhibited by increasingconcentration of anti-CD40L mAb. The concentration of CD40L used is100 ng/mL. The EC50 is 0.215 μg/mL (Routinely tested).
Star Ribbon预染蛋白Marker蛋白质标记物是生物研究和药物开发的重要组成部分。无论是用于蛋白质电泳还是western blot，我们的预染色蛋白质标记物帮助您快速确定目标蛋白质的分子量或评估转移效率。Fc受体蛋白治疗性抗体的功效取决于Fab片段及其对目标抗原的结合活性，还取决于Fc片段及其与关键Fc受体的相互作用。因此，在抗体工程中候选物必须针对一系列受体进行测试。探索我们的重组Fc受体蛋白质的全面收藏！
膜杰作
Star Staining
