近日,Omicron的新变异毒株XBB.1.5引发了广泛的关注。CDC的统计数据表明,美国新增新冠确诊病例中由XBB.1.5引起的比例正在急速攀升。截至到目前为止,其传播速度远超Omicron出现以来的任何一种变异毒株,被WHO认为是迄今为止传播性最强的变种,极有可能引发全球性的新冠浪潮。面对还未大规模到来的XBB,公众保持着高度警惕。
国际 SARS-CoV-2 基因组监测结果[1]
XBB.1.5是重组毒株XBB的最新衍生毒株,最近在美国等国家显示出比此前的流行变异毒株BQ.1.1和XBB.1更强的生长优势。通过对XBB.1.5的基因序列进行分析发现,该突变株相比XBB.1,在Spike蛋白上携带 Ser486Pro 突变,被认为可能是其快速传播的原因。先前的研究表明,相比于Ser486 ,Pro486可能导致Spike蛋白与人类ACE2受体的亲和力增强[2]。近日,北京大学曹云龙、中科院生物物理所王祥喜等人在bioRxiv 发表的题为Enhanced transmissibility of XBB.1.5 is contributed by both strong ACE2 binding and antibody evasion研究结果,通过SPR实验对BQ.1.1、XBB/XBB.1和XBB.1.5的RBD与人ACE2受体亲和力进行检测,结果表明与BQ.1.1和XBB/XBB.1相比,XBB.1.5与人ACE2的结合亲和力明显增强,可能导致改变异毒株获得更强的感染及免疫逃逸能力[3]。
SPR检测SARS-CoV-2 BQ.1.1、XBB/XBB.1和XBB.1.5 RBD
与hACE2 结合亲和力
此外,该团队还通过假病毒中和实验对在BA.1、BA.5或BF.7感染之前接种过3剂 CoronaVac灭活疫苗及BA.5感染前接种过2剂BNT162b2或mRNA-1273 mRNA疫苗的个体的恢复期血浆针对XBB.1.5的中和滴度突破性感染进行了评估。结果显示这些康复患者血浆对XBB.1.5变异毒株的NT50值均显著降低,表明XBB.1.5能够逃逸接种疫苗后突破性感染BA.1、BA.5和BF.7这几种Omicron亚型诱导的中和抗体,也许会导致再次感染的发生[3]。
通过假病毒中和实验对XBB.1.5的免疫逃逸能力进行评估
尽管当前对于XBB.1.5的相关研究尚不完全清晰,然而鉴于其增强的hACE2结合能力剂及较强的抗体逃避能力,增强的受体亲和力是否导致致病性的差异?遇到巨大免疫压力时,其是否会出现与BA.2.75类似的进化趋势而获得额外的免疫逃逸突变?这些问题均需要对XBB.1.5的流行情况进行密切检测,并迅速开展相关研究,同时考虑储备和开发针对XBB.1.5有效的中和抗体及疫苗。
ACROBiosystems百普赛斯XBB.1.5核心研发工具现货发售!
抗原蛋白:
点击货号了解详细信息
分子名 | 货号 | 产品描述 |
Spike RBD | SPD-C5242 | SARS-CoV-2 Spike RBD Protein, His Tag (XBB.1.5/Omicron) |
Spike RBD | SPD-C82Q3 | Biotinylated SARS-CoV-2 Spike RBD Protein, His,Avitag™ (XBB.1.5/Omicron) |
Spike Protein | SPN-C524i | SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) |
ELISA & CBA IgG抗体滴度检测试剂盒:
点击货号了解详细信息
新冠突变 | 货号 | 产品描述 |
XBB.1.5 | RAS-T137 | Human Anti-SARS-CoV-2 (XBB.1.5) Antibody IgG Titer Serologic Assay Kit (Spike Trimer) |
RAS-T138 | Human Anti-SARS-CoV-2 (XBB.1.5) Antibody IgG Titer Serologic Assay Kit (Spike RBD) | |
RAS-T141 | Mouse Anti-SARS-CoV-2 (XBB.1.5) Antibody IgG Titer Serologic Assay Kit (Spike Trimer) | |
RAS-T142 | Mouse Anti-SARS-CoV-2 (XBB.1.5) Antibody IgG Titer Serologic Assay Kit (Spike RBD) | |
XBB.1.5, XBB, BQ.1.1, BQ.1, BA.4&BA.5 |
FCM-B18R | SARS-CoV-2 Omicron Sublineages Total IgG 6-plex Panel (Flow Cytometry Multiplex Bead Assay) |
FCM-B19R | Mouse SARS-CoV-2 Omicron Sublineages Total IgG 6-plex Panel (Flow Cytometry Multiplex Bead Assay) |
验证数据
纯度:经SDS-PAGE验证,XBB.1.5 Spike Trimer(Cat. No. SPN-C524i)纯度>95%,经SEC-MALS验证其三聚体纯度>90%
The purity of SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) (Cat. No. SPN-C524i) on SDS-PAGE is greater than 95% and more than 90% by SEC-MALS. The molecular weight of this protein is around 510-550 kDa.
活性:经验证,XBB.1.5 Spike Trimer(Cat. No. SPN-C524i)与ACE2受体及抗RBD抗体结合活性好,线性范围分别为0.1-4 ng/mL;0.1-1 ng/mL。
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) (Cat. No. SPN-C524i) with a linear range of 0.1-4 ng/mL (QC tested).
Immobilized SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) (Cat. No. SPN-C524i) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No. S1N-M13A1) with a linear range of 0.1-1 ng/mL (Routinely tested).
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参考文献
1. https://github.com/gerstung-lab/SARS-CoV-2-International
2. Starr, T. N. et al. Deep mutational scans for ACE2 binding, RBD expression, and antibody escape in the SARS-CoV-2 Omicron BA.1 and BA.2 receptor-binding domains. PLOS Pathogens 18, e1010951 (2022). https://doi.org:10.1371/journal.ppat.1010951
3. Yue, C., Song, W., Wang, L., Jian, F., Chen, X., Gao, F., Shen, Z., Wang, Y., Wang, X., & Cao, Y. (2023). Enhanced transmissibility of XBB.1.5 is contributed by both strong ACE2 binding and antibody evasion. BioRxiv, 2023.01.03.522427. https://doi.org/10.1101/2023.01.03.522427
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