【满额送】购买免疫检查点蛋白，送免疫检查点抑制剂筛选试剂盒

免疫检查点发展历程中的里程碑

免疫检查点抑制剂筛选试剂盒产品列表

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 μg/mL (Routinely tested).

点击申请Protocol

✍ 高生物活性经BLI验证

Loaded Opdivo (Nivolumab) on ProteinA Biosensor, can bind Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 5.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

点击申请Protocol

✍ 高生物活性经FACS验证

Flow Cytometry assay shows that Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.1 μg/mL (Routinely tested).

点击申请Protocol

✍ PD-1 [Biotinylated] : PD-L1 抑制剂筛选试剂盒(Cat. No. EP-101)

生物素标记的Human PD-1 与PD-L1结合实验

固定2 μg/mL (100 μL/well)的human PD-L1于板孔中，可以结合生物素标记的PD-1，线性区间为0.01 - 0.2 μg/mL。

使用PD-1 [Biotinylated] : PD-L1 抑制剂筛选试剂盒(Cat. No. EP-101)来检测中和性抗体对PD-1-PD-L1结合的抑制效果.

梯度稀释的anti-PD-1 neutralizing antibody (1:1 稀释倍数，从10 μg/mL到0.078 μg/mL )(69.628 nM - 0.544 nM) 加入到PD-L1 : PD-1-Biotin结合的板孔中，得到抑制性曲线。