【Aneuro新品发布】脑类器官及分化试剂盒，为探索武装“脑力”

脑类器官（Cerebral Organoids）是通过将干细胞（通常是诱导多能干细胞，iPSC）培养在特定的培养基中，逐渐分化成神经细胞和神经胶质细胞而形成的复杂的三维结构，类似于胚胎发育过程中的脑组织。脑类器官可以形成具有多种神经细胞类型、神经网络连接和一定活动功能的结构，提供了一种更真实、更可控的模型系统，对神经科学研究具有重要意义，可用于研究人脑的发育、结构和功能，以及神经系统疾病的机制。

脑类器官的应用https://doi.org/10.1007/s12264-023-01065-2

通过观察脑类器官的发育和活动，研究人员可以更好地了解神经细胞的相互作用、神经网络的形成和功能，甚至探索一些神经系统疾病的发病机制，如脑类器官已广泛用于研究神经发育障碍、神经退行性疾病、精神障碍和脑肿瘤。此外，脑类器官还可以用于药物筛选和个体化医疗的研究，研究人员可以将药物添加到脑类器官培养中，评估其对脑组织和功能的影响，从而为药物研发提供更准确的模型。此外，由患者衍生的干细胞可以用于生成个体化的脑类器官，帮助研究特定疾病的发病机制，并评估针对个体患者的治疗方法的有效性。

When using our cerebral organoid differentiation kit, no Matrigel is needed to differentiate iPSCs into cerebral organoids and takes about three months to have abundant cell types including oligodendrocytes, astrocytes, dopaminergic neurons and GABA neurons.

• 长期培养的脑类器官：中心细胞无凋亡

Left: Early-stage cerebral organoid show rosette-like structures (neural stem cells), which become smaller as organoids develop. Right: Day 109 cerebral organoids show uniform morphology and show no dead core inside.

• 早期脑类器官：表达TUJ1神经元标志物

Immunostaining of early-stage Cerebral Organoids

Presence of NESTIN positive cells and positive cells in early stage (day 36) cerebral organoid. TUJ1 (beta III Tubulin), early-stage neuronal marker. NESTIN: a cytoskeletal intermediate filament characterized in neural stem cells.

Immunostaining of day 92 Cerebral Organoids

Presence of TH and MAP2 positive neurons in day 92 cerebral organoid. TH: used as cell marker of dopaminergic neurons. MAP2: mature neuron cell marker.

Immunostaining of Cerebral Organoids show expression of glia cell markers

Left: Presence of GFAP positive cells at day 109 cultured cerebral organoid. Right: Presence of OLIG2 positive cells at day 119 cultured cerebral organoid. GFAP: marker for astrocyte. OLIG2: marker for oligodendrocyte.

Immunostaining of Cerebral Organoids show expression of IBA1 marker

Presence of TH and IBA1 positive cell in day 147 cultured cerebral organoid. IBA1: cell marker for microglia.

RNA-Seq analysis of cerebral organoids at different stages

RNA-Seq analysis of cerebral organoid of day 13, day 44 and day 100, showing the expression of many markers for glutamatergic, dopaminergic, cholinergic, serotonergic and GABAergic neuron. In addition, glia cell markers were also expressed.

Electrophysiology analysis of cerebral organoids

A: patch-clamp recording of excitatory neurons sectioned from cerebral organoids at day 102. Excitatory neuron showed stable response to step injection currents. B: Recording of cerebral organoid (day 60) using silicon probe. Neurons showed spontaneous activities and different waveforms. C: MEA recording indicated spontaneous bursting activities for cerebral organoid (day 86)."

Alpha-synuclein Preformed fibril treatment on Cerebral organoid to model Parkinson’s disease