【ComboX优选】通用型抗体，满足您的不同实验需求

His标签是一种常用的蛋白质纯化标签，由6个组氨酸残基组成。Anti-His抗体可以特异性地识别并结合带有His标签的目标蛋白，常用于Western Blot（WB）、酶联免疫吸附试验（ELISA）、免疫共沉淀（IP）、免疫荧光（IF）、流式细胞术（FC）等实验中。在WB中，Anti-His抗体可以用于检测特定蛋白的表达，而在ELISA中，它可以用于检测His标签蛋白的结合特性。

ELISA验证

Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody, Human IgG1(AY63) (Cat. No. HIS-M535) with a linear range of 0.1-1 ng/mL (QC tested).

Western Blot验证

Detection of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb by Western Blot. Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb at 1/20000 dilution + Human LAG-3 / CD223 Protein , His Tag at 75-250ng. Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.

HRP（辣根过氧化物酶）标记的Anti-His抗体是一种可直接应用于WB等实验的化学发光检测工具。在底物存在的情况下，该HPR标记抗体能够通过酶促反应产生强烈的发光信号，从而实现对目标蛋白的高灵敏度检测。这种发光信号可以被成像设备轻松捕捉，使得实验过程既简便又高效，特别适用于低丰度蛋白的检测。

ELISA验证

Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).

Western Blot验证

Detection of HRP conjugated Anti-His Tag Antibody (AY63), mAb by Western Blot. HRP conjugated Anti-His Antibody (AY63), mAb at 1/10000 dilution + Human LAG-3 / CD223 Protein, His Tag at 75-250ng. Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.

生物素（Biotin）标记的Anti-His抗体是另一种增强检测信号的抗体工具。Biotin与链霉亲和素（Streptavidin）之间具有极高的亲和力，因此，当使用Biotin标记的抗体后，可以通过加入Streptavidin标记的报告分子（如荧光染料或酶）来进一步放大信号。这种方法适用于多种检测领域，包括WB、ELISA、FACS等。

ELISA验证

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BQ.1/Omicron) (Cat. No. NUN-C52Hx) at 1μg/mL (100μL/well) can bind Biotinylated Anti-His Tag Antibody (AY63), Fab (Cat. No. HIS-BVM539) with a linear range of 0.2-100ng/mL (QC tested).

聚乙二醇（PEG）是一常见的药用辅料，具有无毒、无免疫原性、水溶性好等特点，广泛应用于蛋白质、肽、小分子等药物的修饰中。经PEG修饰后可增强药物的稳定性和溶解性，提高药物半衰期，减少给药次数等。Anti-PEG抗体能够特异性识别PEG分子，可用于研究PEG化药物的体内吸收、分布、代谢及排泄。我们的Anti-PEG抗体具有高特异性和亲和力，可识别不同分子量的PEG，适用于ELISA、WB等实验，助力PEG化药物的药代动力学研究。

SDS-PAGE验证

Monoclonal Anti-PEG Antibody, Human IgG1 (5G3-1C6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

ELISA验证

Immobilized PEG Coated Plates, Clear, 12×8-Well Strips, White Frame at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PEG Antibody, Human IgG1 (5G3-1C6) (Cat. No. PEG-M731) with a linear range of 0.004-0.125 μg/mL (QC tested).

Immobilized 5KD-PEG/20KD-PEG/40KD-PEG at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) (Cat. No. PEG-M687) with a linear range of 5-78 ng/mL (Routinely tested).

MSD验证

Immobilized Biotin Human IFN on MSD GoldTM 96-well Streptavidin Plate,can bind anti-IFN-PEG when detected by Sulfo Anti-PEG Antibody.Use the MESO QuickPlex SQ 120 MM instrument for analysis.

Anti-Human IgG Fc抗体可以特异性识别Human IgG Fc区域，该抗体用于检测或捕获人源性IgG抗体、人Fc融合蛋白等，是研究自身免疫疾病和开发抗体疗法不可或缺的工具。

ELISA验证

Anti-SARS-CoV-2 Spike RBD antibodies of various subclasses (IgG2, IgG3, IgG4), along with the human ACE2 protein, effectively bind to the monoclonal Anti-Human-IgG-Fc antibody. However, they do not interact with the Anti-SARS-CoV-2 Spike RBD chimeric mAb of the IgA1 subclass or the Human CD19 protein.

类似于HRP-Anti-His抗体，将HRP与Anti-Human IgG Fc抗体结合，用于增强检测信号，该抗体可直接应用于WB、ELISA等实验中，能够提高实验的可靠性和准确性。

产品推荐：HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Cat. No. IGG-LY69)

ELISA验证

Immobilized Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) at 2 μg/mL (100 μL/well) can bind Human Monoclonal Anti-TNF-alpha antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) when detected by HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8),mAb (Cat. No. IGG-LY69) dilute at 1:10000 (0.0842μg/ml) (QC tested).