Recombinant Proteins iso9001 ISO13485
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B7-CD28家族:免疫调节中重要协同刺激途径

背景
T细胞活化不仅需要抗原特异性T细胞受体 (TCR)与抗原递呈细胞 (APC)上的抗原肽 MHC分子复合物的相互作用作为第一信号,还需要协同刺激作为第二信号。在这些协同刺激信号途径中,B7-CD28超家族是目前发现的共刺激分子家族之一,结构多为Ⅰ型跨膜糖蛋白,属于免疫球蛋白超家族。这些协同刺激途径在免疫调节过程中发挥重要作用,既可以提供激动性信号增强和维持T细胞免疫应答,也可以通过抑制性信号减弱T细胞免疫应答。因此B7-CD28超家族在肿瘤免疫治疗、自身免疫中发挥重要作用。B7家族成员包括B7-1、B7-2、B7-H1、B7-DC、B7-H2、B7-H3、B7-H4、B7-H5、BTNL2、B7-H6和B7-H7。
为满足市场需求,ACROBiosystems优化开发了一系列高纯度、高生物活性经多平台验证的B7家族蛋白,适用于大分子抗体药物的免疫、SPR、细胞活性检测等实验,可加速抗体药物的开发进程。
B7家族成员列表
B7配体家族 别称 表达 对应受体 在T / NK细胞反应中的作用
免疫细胞 肿瘤
B7-H1HOT

PD-L1,CD274,PDCD1LG1

+ + PD-1HOT,CD80 抑制
B7-DC

PD-L2,CD273,PDCD1LG2

+ + PD-1,RGMB 抑制
B7-1 HOT

CD80,BB1,LAB-7,CD28LG1

+ CD28HOT,CTLA-4HOT, PD-L1HOT 激活/抑制
B7-2 HOT

CD86,B70,LAB72,CD28LG2

+ CD28HOT,CTLA-4HOT 激活/抑制
B7-H3 HOT

CD276,B7RP-2

+ + Unidentified 激活/抑制
B7-H5

VISTA,GI24,VSIR,SISP1,PD1H,DD1α

+ - VSIG3 抑制
B7-H2

ICOS-L,ICOSLG,GL50,B7RP1,LICOS

+ ICOS,CD28HOT,CTLA-4HOT 激活
B7-H4

B7x,B7S1,B7h.5,VTCN1

+ + Unidentified 抑制
B7-H6

NCR3LG1,NR3L1

- + NKp30 激活
B7-H7

HHLA2,B7y

+ + CD28H 激活/抑制
产品列表
  • 抑制
  • 激活
  • 激活/抑制
分子名 货号 物种 产品描述
B7-H1(PD-L1)PD1-C5253Cynomolgus / Rhesus macaqueCynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, Fc Tag
PD1-C52H3CanineCanine PD-L1 / B7-H1 Protein, Fc Tag
PD1-C52H4Cynomolgus / Rhesus macaqueCynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, His Tag
PD1-H5229HumanHuman PD-L1 / B7-H1 Protein, His Tag (MALS verified)
PD1-H5258HumanHuman PD-L1 / B7-H1 Protein, Fc Tag (MALS verified)
PD1-H5282HumanHuman PD-L1 / B7-H1 Protein, Strep Tag
PD1-H52A3HumanHuman PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified)
PD1-H52H3HumanHuman PD-L1 / B7-H1 (19-134) Protein, His Tag (MALS verified)
PD1-H82A3HumanBiotinylated Human PD-L1 / B7-H1 Protein, Mouse IgG2a Fc,
PD1-H82E5HumanBiotinylated Human PD-L1 / B7-H1 Protein, ,His Tag (recommended for biopanning, MALS verified)
PD1-H82F3HumanBiotinylated Human PD-L1 / B7-H1 Protein, Fc,,His Tag (MALS verified)
PD1-M5220MouseMouse PD-L1 / B7-H1 Protein, His Tag
PD1-M5251MouseMouse PD-L1 / B7-H1 Protein, Fc Tag
PD1-M52A2MouseMouse PD-L1 / B7-H1 Protein, Mouse IgG2a Fc Tag, low endotoxin
PD1-M82F5MouseBiotinylated Mouse PD-L1 / B7-H1 Protein, Fc,
PDL-H5250HumanHuman PD-L1 / B7-H1 Protein, Llama IgG2b Fc Tag, low endotoxin
PDL-H82E4HumanBiotinylated Human PD-L1 / B7-H1 (19-134) Protein, His, (recommended for biopanning) (MALS verified)
PDL-H82F2HumanBiotinylated Human PD-L1 / B7-H1 Protein, Fc, (MALS verified)
PDL-R52H6RabbitRabbit PD-L1 / B7-H1 Protein, His Tag
B7-H5B75-C5220Rhesus macaqueRhesus macaque B7-H5 / Gi24 / VISTA Protein, His Tag
B75-H5258HumanHuman B7-H5 / Gi24 / VISTA Protein, Mouse IgG2a Fc Tag, low endotoxin
B75-H5259HumanHuman B7-H5 / Gi24 / VISTA Protein, Fc Tag
B75-H52H0HumanHuman B7-H5 / Gi24 / VISTA Protein, His Tag (MALS verified)
B75-H82E1HumanBiotinylated Human B7-H5 / Gi24 / VISTA Protein, ,His Tag
B75-H82F3HumanBiotinylated Human B7-H5 / Gi24 / VISTA Protein, Fc,
B75-M52H7MouseMouse B7-H5 / Gi24 / VISTA Protein, His Tag
B7-DC(PD-L2)PD2-H5220HumanHuman PD-L2 / B7-DC Protein, His Tag (SPR verified)
PD2-H5251HumanHuman PD-L2 / B7-DC Protein, Fc Tag (HPLC verified)
PD2-H52A5HumanHuman PD-L2 / B7-DC Protein, mouse IgG1 Fc Tag
PD2-H82E8HumanBiotinylated Human PD-L2 / B7-DC Protein, ,His Tag (recommended for biopanning)
PD2-H82F6HumanBiotinylated Human PD-L2 / B7-DC Protein, Fc,
PD2-M5254MouseMouse PD-L2 / B7-DC Protein, Fc Tag
PD2-M52E3MouseMouse PD-L2 / B7-DC Protein, His Tag
B7-H4B74-H5222HumanHuman B7-H4 Protein, His Tag
B74-H5256HumanHuman B7-H4 Protein, Fc Tag, low endotoxin
B74-H8222HumanBiotinylated Human B7-H4 Protein, His Tag, ultra sensitivity (primary amine labeling)
B74-H82E2HumanBiotinylated Human B7-H4 Protein, ,His Tag (recommended for biopanning)
分子名 货号 物种 产品描述
B7-H2B72-C525bCynomolgusCynomolgus B7-H2 / ICOSLG Protein, Fc Tag
B72-C52HbCynomolgusCynomolgus B7-H2 / ICOSLG Protein, His Tag
B72-H5221HumanHuman B7-H2 / ICOSLG Protein, His Tag
B72-H5254HumanHuman B7-H2 / ICOSLG Protein, Fc Tag
B72-M5258MouseMouse B7-H2 / ICOSLG Protein, Fc Tag
B72-M52H7MouseMouse B7-H2 / ICOSLG Protein, His Tag
B7-H6B76-C52HaCynomolgusCynomolgus B7-H6 / NCR3LG1 Protein
B76-H52H8HumanHuman B7-H6 / NCR3LG1 Protein, His Tag
B76-H82E5HumanBiotinylated Human B7-H6 / NCR3LG1 Protein, His,
B76-H82WbHumanBiotinylated Human B7-H6 / NCR3LG1 Protein, Fc,
分子名 货号 物种 产品描述
B7-1B71-H5228HumanHuman B7-1 / CD80 Protein, His Tag
B71-H5259HumanHuman B7-1 / CD80 Protein, Fc Tag (HPLC-verified)
B71-H52A4HumanHuman B7-1 / CD80 Protein, Mouse IgG2a Fc Tag, low endotoxin
B71-H82E9HumanBiotinylated Human B7-1 / CD80 Protein, ,His Tag (recommended for biopanning)
B71-H82F2HumanBiotinylated Human B7-1 / CD80 Protein, Fc,
CD0-C5252Cynomolgus / Rhesus macaqueCynomolgus / Rhesus macaque B7-1 / CD80 Protein, Fc Tag
CD0-C52H3Cynomolgus / Rhesus macaqueCynomolgus / Rhesus macaque B7-1 / CD80 Protein, His Tag (HPLC-verified)
CD0-M5228MouseMouse B7-1 / CD80 Protein, His Tag
CD0-M5259MouseMouse B7-1 / CD80 Protein, Fc Tag
B7-2CD6-C5254Cynomolgus / Rhesus macaqueCynomolgus / Rhesus macaque B7-2 / CD86 Protein, Fc Tag
CD6-C52H5Cynomolgus / Rhesus macaqueCynomolgus / Rhesus macaque B7-2 / CD86 Protein, His Tag (HPLC-verified)
CD6-H5223HumanHuman B7-2 / CD86 Protein, His Tag
CD6-H5257HumanHuman B7-2 / CD86 Protein, Fc Tag
CD6-H82F5HumanBiotinylated Human B7-2 / CD86 Protein, Fc,
CD6-M5251MouseMouse B7-2 / CD86 Protein, Fc Tag
CD6-M52H0MouseMouse B7-2 / CD86 Protein, His Tag
B7-H3B73-C52HaCynomolgusCynomolgus B7-H3 / CD276 Protein, His Tag (MALS verified)
B73-H5253HumanHuman B7-H3 / CD276 Protein, Fc Tag (MALS verified)
B73-H52E2HumanHuman B7-H3 / CD276 Protein, His Tag (MALS verified)
B73-H82E6HumanBiotinylated Human B7-H3 / CD276 Protein, ,His Tag
B73-H82F5HumanBiotinylated Human B7-H3 / CD276 Protein, Fc, (MALS verified)
B73-M5255MouseMouse B7-H3 / CD276 Protein, Fc Tag (MALS verified)
B73-M52H4MouseMouse B7-H3 / CD276 Protein, His Tag (MALS verified)
B7-H7B77-C52H3CynomolgusCynomolgus B7-H7 / HHLA2 Protein, His Tag
B77-H5257HumanHuman B7-H7 / HHLA2 Protein, Fc Tag
B77-H52H5HumanHuman B7-H7 / HHLA2 Protein, His Tag
B77-H82F5HumanBiotinylated Human B7-H7 / HHLA2 Protein, Fc,
验证数据
MALS验证高纯度

The purity of Human B7-H3, His Tag (Cat. No. B73-H52E2) was more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

批间一致性高
针对药物开发各应用场景开发
场景1:抗体药物筛选

Immobilized Human ICOS, Fc Tag (Cat. No. ICS-H5258) at 1 μg/mL (100 μL/well) can bind Human B7-H2, His Tag (Cat. No. B72-H5221) with a linear range of 5-78 ng/mL (QC tested).

Protocol

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 μg/mL (Routinely tested).

Protocol

场景2:抗体亲和力验证

Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

场景3:细胞活性验证

FACS

Flow Cytometry assay shows that Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) can bind to 293 cell overexpressing human CTLA-4. The concentration of Human B7-1 is 0.3 μg/mL (Routinely tested).

Protocol

FACS analysis shows that the binding of Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) to 293 overexpressing CTLA-4 was inhibited by increasing concentration of neutralizing Anti-Human CTLA-4 antibody. The concentration of B7-1 used is 0.3 μg/mL. The IC50 is 3.025 μg/mL (Routinely tested).

Protocol

Cell based assay

Human B7-H3 (4Ig), His Tag (Cat. No. B7B-H52E7) inhibits Anti-CD3-induced proliferation of PBMC. The EC50 for this effect is 7.95 μg/mL (Routinely tested).

Protocol

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with an EC50 of 0.029 μg/mL (Routinely tested).

Protocol

产品评论
ACROBiosystems提供的高质量B7系列蛋白产品客户反馈良好。
 
评论(12)
  1. 138XXXXXXX2
  2. 5人赞
  3. 用过很多种类Acro的重组蛋白,包括一些热门的靶点抗原,像LAG3、B7H3等,还有一些重组的细胞因子像IL-36α/β/γ等。说一下体验吧,外包装盒很酷炫的感觉喔~靶点抗原作为ELISA包被原料还是很好用的,跟抗体结合活性高,特异性也很强,唯一“不足”的地方可能就是价格有点子小贵吧。细胞因子类的用作过细胞功能学方面的检测,虽然刺激效果也不错,但是有些跟R&D的刺激效果比起来还是有一定改善空间,希望Acro能不断提升产品性能,越做越好!
  4. 2020-01-17
  1. 182XXXXXXX2
  2. 1人赞
  3. Fc标签的B7H2的SEC纯度确实不错,可以看到,单体的纯度有91%,另外,还有些聚集体,对于一个抗原,这个纯度很不错。
  4. >
  5. 2020-01-10
  1. 158XXXXXXX6
  2. 2人赞
  3. PDL1抗原买回来进行生物学活性试验,包括Elisa等,买回来后还进行了关键批次的桥接,该抗原纯度较好,跟其他商家的同类抗原比起来效果好一些。
  4. 2019-10-23
  1. 158XXXXXXX6
  2. 3人赞
  3. human PD-L1 的纯度经过SEC-HPLC检测,有很高的纯度,购买还产品用于公司产品的检测,各方面都很好,产品质量不错物流也棒棒哒,acro好多产品都经常在用,纯度都很好哦
  4. 2019-10-18
  1. 152XXXXXXX2
  2. 2人赞
  3. 使用过该蛋白做ELISA实验,将CD80蛋白包被到孔板中,然后将抗体分子与该biotin-PDL1蛋白混合做blocking assay,可以获得理想的数据。
  4. >
  5. 2019-10-12

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