STAT通路与疾病发生 - ACROBiosystems百普赛斯生物科技股份有限公司

信号通路（Signal pathway）是指能将细胞外的分子信号经细胞膜传入细胞内发挥效应的一系列酶促反应通路。一个信号通路的构成主要包括三部分元件，即受体（receptor）与配体（ligand）、蛋白激酶（kinase）、转录因子。在信号传导过程中，配体与细胞膜表面或细胞内受体结合，蛋白激酶通过磷酸化改变下游蛋白构象从而对一系列下游蛋白进行调节并将信号级联放大，最终通过转录因子对基因转录进行调节最终产生一系列细胞的综合应答。信号传导过程是细胞对外界刺激产生反应并最终引发特异性生物学效应的有效方式。几十年的基础研究逐渐解释了许多在癌症、心血管疾病、自身免疫性疾病等疾病发生与发展过程中起关键作用的信号通路。同时，针对信号通路的药物筛选与开发也成为了疾病免疫治疗的新思路。

JAK-STAT信号传送途径是一个进化保守的信号级联反应，介导对细胞因子和生长因子的反应，使得细胞外的化学信号跨越细胞膜并将信息传送到细胞核内DNA上的基因启动子，最终引起细胞中DNA转录与活性水平发生改变，是除了第二信使系统外最重要的信号途径。JAK-STAT系统由三个主要部分组成：受体、JAK激酶、信号转导及转录激活蛋白（STAT）。

ACRO基于充分的信号通路研究，结合靶向信号通路的药物作用机制，开发了经典信号通路JAK/STAT相关产品，提供包括细胞因子、生长因子等信号分子、细胞膜表面受体及配体、蛋白激酶、转录因子等靶点蛋白，同时拓展有包括预偶联磁珠、抑制剂筛选试剂盒、报告基因细胞系等更多产品类型，力求为信号通路研究及靶向信号通路的药物开发提供一站式高效解决方案。

热门通路

JAK/STAT5

信号通路：肺和肠道上皮细胞和角质形成细胞收到刺激后释放TSLP，与其受体TSLPR结合形成二聚体后募集IL-7Rα，形成三元TSLPR-TSLP-IL-7Rα复合物，进而通过JAK和STAT5启动促炎信号。

相关疾病：过敏性疾病、慢性炎症、自身免疫性疾病、癌症等。

热门产品：TSLP、TSLPR、IL-7 R alpha、IL-7 R alpha & TSLP R ^Hot、TSLP抑制剂筛选试剂盒、TSLPR报告基因细胞系^New。

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JAK/STAT3

信号通路：JAK激酶是许多细胞因子、生长因子及干扰素的重要信号传感器。在某些应激反应条件下，一些细胞因子与相应的受体结合，诱导JAK磷酸化，从而使STAT3活化，促使STAT3的二聚化、核易位、与特定的DNA序列结合，行使基因转录调控的功能。

相关疾病：肿瘤、免疫类疾病、感染类疾病等。

热门产品：细胞因子、生长因子、JAK1 ^New、STAT3^New、EGFR预偶联磁珠、EGFR报告基因细胞系、STAT3报告基因细胞系。

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JAK/STAT5

免疫/结合验证——靶点蛋白&受体
抗体/抑制剂筛选——抑制剂筛选试剂盒
细胞水平功能验证——报告基因细胞系

靶点蛋白：

种属齐全：覆盖Human、Mouse、Cynomolgus不同种属；
标签选择丰富：提供His、His & Avi、Fc、mFc、Fc & Fc不同产品形式；
模拟天然构象：除TSLP、TSLP R、IL-7 R外，特别推出行业新品TSLP R & IL7 R异源二聚体，真实模拟天然构象；
纯度及活性保证：纯度经SDS-PAGE、MALS验证，活性经ELISA、SPR、BLI等多平台验。

产品列表

分子	货号	物种	产品描述	订购/预购
TSLP	TSP-C52H4	Cynomolgus	Cynomolgus TSLP (R127A, R130S) Protein, His Tag	订购
	TSP-C52H8	Cynomolgus	Cynomolgus TSLP Protein, His Tag	订购
	TSP-C82E3	Cynomolgus	Biotinylated Cynomolgus TSLP Protein, His,Avitag™	订购
	TSP-H5243	Human	Human TSLP Protein (R127A, R130A), His Tag (MALS verified)	订购
	TSP-H5255	Human	Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag	订购
	TSP-H52Ha	Human	Human TSLP (R127A, R130A) Protein, His Tag (MALS verified)	订购
	TSP-H52Hb	Human	Human TSLP Protein, His Tag, premium grade	订购
	TSP-H82E0	Human	Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag™ (MALS verified)	订购
	TSP-H82Eb	Human	Biotinylated Human TSLP Protein, His,Avitag™	订购
	TSP-M52H8	Mouse	Mouse TSLP Protein, His Tag (MALS verified)	订购
	TSP-M82E3	Mouse	Biotinylated Mouse TSLP Protein, His,Avitag™	订购
	TSP-R52H3	Rat	Rat TSLP Protein, His Tag	订购
TSLP R	TSR-H525a	Human	Human TSLP R Protein, Fc Tag	订购
IL-7 R alpha & TSLP R	ILR-H5255	Human	Human IL-7 R alpha&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)	订购
IL-7 R alpha & TSLP R	ILR-H525x	Human	Human IL-7 R alpha&TSLP R Heterodimer Protein, Fc Tag&Fc Tag	订购
IL-7 R alpha	IL7-H5258	Human	Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag	订购
	IL7-H52H7	Human	Human IL-7 R alpha / CD127 Protein, His Tag	订购
	IL7-H82F9	Human	Biotinylated Human IL-7 R alpha / CD127 Protein, Fc,Avitag™	订购
	ILA-H525a	Human	Human IL-7 R alpha / CD127 Protein, Fc Tag	订购
	ILR-M5253	Mouse	Mouse IL-7 R alpha / CD127 Protein, Fc Tag	订购

结合活性验证——TSLP蛋白及其受体

TSLP R & IL7 R异源二聚体纯度经MALS验证>85%

The purity of Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) is greater than 95% verified by SDS-PAGE. The purity of the heterodimer is more than 85% and the molecular weight of this protein is around 125-140 kDa verified by SEC-MALS.

Protocol

TSLP R & IL7 R异源二聚体可用于验证抗体与TSLP结合活性

Serial dilutions of Monoclonal Anti-Human TSLP Antibody, Human IgG2 were added into Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255): Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) binding reactions. The half maximal inhibitory concentration (IC50) is 1.09688 μg/mL (Routinely tested).

Protocol

抑制剂筛选试剂盒：

快速，高通量，可在2h内完成90+样本检测；
严格指控，保证试剂盒批间差及精密度均<10%；
预包被模拟天然构象的TSLPR和IL-7R异源二聚体，提高筛选成功率。

产品列表

货号	产品描述	订购/预购
EP-129	TSLP[Biotinylated]:IL-7Rα & TSLP R Inhibitor Screening ELISA Kit	订购

抗体/抑制剂筛选——抑制剂筛选试剂盒

Serial dilutions of Anti-TSLP Neutralizing antibody (Catalog # EP129-C03) (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

Protocol

功能细胞系：

代次稳定性好，10-26代传代稳定性经验证；
检测窗口大，适用不同药效筛选；
反应信号强，确保高灵敏度；
基于明确MOA设计，可用于药物开发过程中的细胞功能性验证实验及CMC质控放行。

产品列表

货号	产品描述	订购/预购
CHEK-ATF045	TSLPR (Luc) HEK293 Reporter Cell	订购

细胞水平功能验证——报告基因细胞系

细胞性质验证

Co-expression analysis of human TSLPR and IL7Rα on TSLPR (Luc) HEK293 Reporter Cell by FACS. Cell surface staining was performed on TSLPR (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-TSLPR antibody and FITC-labeled anti-IL7Rα antibody.

Protocol

Response to human TSLP protein (RLU). The TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein (Cat. No. TSP-H52Hb). The EC50 was approximately 0.1392 μg/mL.

Protocol

Response to human TSLP protein (Fold). The TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein (Cat. No. TSP-H52Hb). The max induction fold was approximately 45.15.

Protocol

代次稳定性验证

Passage stability analysis of receptors expression by FACS. Flow cytometry surface staining of human TSLPR and IL7Rα on TSLPR (Luc) HEK293 Reporter Cell demonstrates consistent mean fluorescent intensity across passage10-26. (A) Human IL7Rα expression analysis. (B) Human TSLPR expression analysis.

Protocol

Passage stability analysis by Signaling Bioassay. The continuously growing TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein. Human TSLP protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-26.

Protocol

应用案例

Inhibition of human TSLP protein-induced reporter activity by anti-human TSLP neutralizing antibody. This reporter cell was incubated with serial dilutions of antibodies in the presence of human TSLP protein (Cat. No. TSP-H52Hb) with a final concentration of 0.3 μg/mL. The EC50 of anti-human TSLP neutralizing antibody is approximately 0.55 μg/mL.

Protocol

JAK/STAT3

免疫/结合验证——靶点蛋白&受体
抗体/抑制剂筛选——预偶联磁珠
细胞水平功能验证——报告基因细胞系

靶点蛋白：

胞内胞外靶点丰富：覆盖STAT3经典信号通路内外靶点蛋白，包括EGF、EGR R、IL-6、IL-6 R alpha、JAK1、STAT3等；
种属选择多样：覆盖Human、Mouse、Cynomolgus不同种属；
标签选择丰富：提供His、His & Avi、Fc、mFc、Fc & Fc不同产品形式；
纯度活性保证：纯度经SDS-PAGE、MALS验证>90%，与降解剂结合活性经BLI、SPR等实验方法验证。

产品列表

分子	货号	物种	产品描述	订购/预购
EGF	EGF-H525b	Human	Human EGF Protein, Mouse IgG2a Fc Tag	订购
	EGF-H52H3	Human	Human EGF Protein, His Tag, premium grade	订购
	EGF-M5265	Mouse	Mouse EGF Protein, Fc Tag	订购
EGF R	EGR-C5252	Rhesus macaque	Rhesus macaque EGF R Protein, Fc Tag (MALS verified)	订购
	EGR-C52H1	Rhesus macaque	Rhesus macaque EGF R Protein, His Tag	订购
	EGR-H5222	Human	Human EGF R Protein, His Tag (MALS verified)	订购
	EGR-H522a	Human	Human EGF R Protein, His Tag, low endotoxin (MALS verified)	订购
	EGR-H5252	Human	Human EGF R Protein, Fc Tag (MALS verified)	订购
	EGR-H5259	Human	Human EGF R Protein, Llama IgG2b Fc Tag, low endotoxin	订购
	EGR-H82E3	Human	Biotinylated Human EGF R Protein, His,Avitag™ (MALS verified)	订购
	EGR-H82F8	Human	Biotinylated Human EGF R Protein, Fc,Avitag™ (MALS verified)	订购
	EGR-HA2H8	Human	APC-Labeled Human EGF R Protein, His TagStar Staining	订购
	EGR-HF256	Human	FITC-Labeled Human EGF R Protein, Fc Tag	订购
	EGR-HF2H5	Human	FITC-Labeled Human EGF R Protein, His Tag	订购
	EGR-HP2E3	Human	PE-Labeled Human EGF R Protein, His Tag (Site-specific conjugation)	订购
	EGR-HP2H7	Human	PE-Labeled Human EGF R Protein, His TagStar Staining	订购
	EGR-M5224	Mouse	Mouse EGF R Protein, His Tag	订购
	EGR-M5257	Mouse	Mouse EGF R Protein, Fc Tag (MALS verified)	订购
	EGR-M82E3	Mouse	Biotinylated Mouse EGF R Protein, His,Avitag™ (MALS verified)	订购
	EGR-M82F3	Mouse	Mouse EGF R Protein, Fc,Avitag™ (MALS verified)	订购
	EGR-R52H7	Rabbit	Rabbit EGF R Protein, His Tag	订购
IL-6	GMP-L06H27	Human	GMP Human IL-6 Protein	订购
	IL6-C5249	Cynomolgus	Cynomolgus IL-6 Protein, His Tag (MALS verified)	订购
	IL6-H4218	Human	Human IL-6 Protein, premium grade	订购
	IL6-H5243	Human	Human IL-6 Protein, His Tag (MALS verified)	订购
	IL6-H8218	Human	Biotinylated Human IL-6 Protein, epitope tag free, ultra sensitivity, primary amine labeling	订购
	IL6-H82Q9	Human	Biotinylated Human IL-6 Protein, Avitag™,His Tag (MALS verified)	订购
	IL6-M5245	Mouse	Mouse IL-6 Protein, His Tag	订购
	IL6-M82Q7	Mouse	Biotinylated Mouse IL-6 Protein, Avitag™,His Tag	订购
IL-6 R alpha	CD6-H82E8	Human	Biotinylated Human IL-6 R alpha / CD126 Protein, Avitag™,His Tag (MALS verified)	订购
	ILR-H4223	Human	Human IL-6 R alpha / CD126 Protein, His Tag	订购
	ILR-H5259	Human	Human IL-6 R alpha / CD126 Protein, Fc Tag	订购
	ILR-H82F9	Human	Biotinylated Human IL-6 R alpha / CD126 Protein, Fc,Avitag™	订购
	ILR-M5255	Mouse	Mouse IL-6 R alpha / CD126 Protein, Fc Tag (MALS verified)	订购
	ILR-M82E9	Mouse	Biotinylated Mouse IL-6 R alpha / CD126 Protein, His,Avitag™	订购
gp130	ILT-M5252	Mouse	Mouse gp130 / CD130 / IL-6 R beta Protein, Fc Tag	订购
gp130	ILT-M52H1	Mouse	Mouse gp130 / CD130 / IL-6 R beta Protein, His Tag	订购

结合活性验证——TSLP蛋白及其受体

人源STAT3蛋白(Cat. No. ST3-H5149) 纯度经MALS验证>90%

The purity of Human STAT3, His Tag (Cat. No. ST3-H5149) is more than 95% verified by SDS-PAG and greater than 90% verified by SEC-MALS. The molecular weight of this protein is around 60-75 kDa.

Protocol

人源STAT3蛋白与其降解剂结合活性经SPR验证

Human STAT3, His Tag (Cat. No. ST3-H5149) immobilized on CM5 Chip can bind STAT3 degrader with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

预偶联磁珠：

采用生物素化蛋白-链霉亲和素磁珠系统，饱和浓度抗原，保证充分包被；
磁珠粒径均匀、比表面积大，捕获效率高，结果重复性好。

产品列表

货号	产品描述	订购/预购
MBE-K012	Human EGFR-coupled Magnetic Beads	订购

抗体/抑制剂筛选——预偶联磁珠

Immobilized 35 μg EGFR protein to 1 mg Beads, can bind the Anti-EGFR Antibody (Cetuximab) with an EC50 of 0.737 μg/mL (QC tested).

Protocol

功能细胞系：

基于明确MOA设计，提供EGFR/STAT3和IL-6/JAK/STAT3通路报告基因细胞系；
代次稳定性好，可稳定传代10-20代；
检测窗口大，适用不同药效筛选；
反应信号强，确保高灵敏度；
可用于靶向该信号通路的小分子抑制剂与抗体药筛选及细胞功能验证实验。

产品列表

货号	产品描述	订购/预购
CHEK-ATF049	EGFR (Luc) HEK293 Reporter Cell	订购
CHEK-ATF047	STAT3 (Luc) HEK293 Reporter Cell	订购

细胞水平功能验证——报告基因细胞系

细胞性质验证

Expression analysis of human EGFR on EGFR (Luc) HEK293 Reporter Cell by FACS. Cell surface staining was performed on EGFR (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-EGFR antibody.

Protocol

Response to human EGF protein (RLU). The EGFR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein (Cat. No. ST3-H5149). The EC50 was approximately 56.23 ng/mL.

Protocol

Response to human EGF protein (Fold). The EGFR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein (Cat. No. ST3-H5149). The max induction fold was approximately 56.

Protocol

代次稳定性验证

Protocol

应用案例

Inhibition of human EGF protein-induced reporter activity by human EGFR small molecule inhibitor. This reporter cell was incubated with serial dilutions of inhibitors in the presence of human EGF protein (Cat. No. ST3-H5149) with a final concentration of 50 ng/mL. The EC50 of human EGFR small molecule inhibitor (Erlotinib) was approximately 0.01 μM.

Protocol

免疫检查点蛋白

细胞因子靶点

GMP级别产品

细胞疗法靶点

Enzymes

全长多次跨膜蛋白

脑与神经蛋白

肿瘤微环境蛋白

信号通路蛋白

其它蛋白

免疫组化 (IHC) 抗体

抗独特型抗体

同型对照

通用型抗体

其他抗体

抗体服务

GMP级别生长因子

细胞激活

细胞培养添加剂

其他产品

ELISA

qPCR试剂盒

流式细胞术

ADC 偶联试剂盒

免疫组化（IHC）试剂盒

MPCLIA

其它试剂盒

新冠病毒

水痘-带状疱疹病毒

呼吸道合胞病毒

狂犬病毒

其他传染病相关试剂

ViruStop检测服务

iPSC分化的即用型活体类器官

类器官分化试剂盒

类器官培养试剂

类器官技术服务

类器官病理检测服务

实验试剂

功能细胞株 (Cellinsight)

神经科学在体电生理实验解决方案

实验仪器设备

CAR靶点蛋白

其它靶点蛋白

通用型蛋白

药物筛选与分析服务

伴随诊断

细胞培养产品

Premium(Pre-GMP) & GMP 级别原材料

基因编辑产品

大规模生产

核酸去除产品

CAR检测工具

效力测定

残留检测试剂盒 - resDetect

药代动力学研究

免疫原性研究

药效学研究

审计&申报文件支持

毒理研究

伴随诊断

药代动力学研究

免疫原性研究

毒性研究

基因编辑

AAV颗粒滴度定量检测试剂盒

伴随诊断

Premium(Pre-GMP) & GMP 级别原材料

核酸去除

基因编辑

AAV颗粒滴度定量检测试剂盒

大规模生产

残留检测 - resDetect

效力测定

药效学研究

审计&申报文件支持

毒性研究

伴随诊断

毒性研究

靶点蛋白