MHC-多肽复合物定制服务

5e5 of anti-NY-ESO-1 TCR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein (Cat. No. HL1-HP2E5) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

5e5 of anti-NY-ESO-1 TCR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human HLA-A0201&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (Cat. No. HL1-HP2E6) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

Performance of PE-labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer from ACROBiosystems (Cat. No. HL1-HP2E5) and other brand were evaluated by flow cytometry under the manufacturer’s recommended conditions. ACROBiosystems’ PE-Labeled NY-ESO-1 Tetramer Protein shows 3x higher MFI signal strength than competitor M, revealing much better TCR binding.

Performance of PE-labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer from ACROBiosystems (Cat. No. HL1-HP2E6) and other brand were evaluated by flow cytometry under the manufacturer’s recommended conditions. ACROBiosystems’ PE-Labeled NY-ESO-1 Tetramer Protein shows 2x higher MFI signal strength than competitor M, revealing much better TCR binding.

The purity of Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Complex Protein (Cat. No. HL1-H82E6) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

The complex protein was verified stable after incubation under 37 degrees for 24 hours, and no significant dissociation of HLA-A*2402 and B2M(Cat. No. HLM-H82W4) was observed from HPLC-SEC analysis.

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein (MALS verified) (Cat. No. HL1-H52E8) is stable in undiluted samples at 37℃ for 48 h and freeze-throw cycles are stable without performance reduction.

Biotinylated Human HLA-A*02:01&B2M Complex Protein (Peptide free, MALS verified) (Cat. No. HLM-H82W3) is stable in undiluted samples at 37℃ for 48 h and freeze-throw cycles are stable without performance reduction.