血液瘤CAR靶点

The purity of Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Immobilized FMC63 (Mouse IgG2a) at 1 μg/mL (100 μL/well) can bind Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) with a linear range of 1-31 ng/mL (QC tested).

Immobilized Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) at 5 μg/mL (100 μL/well) can bind FMC63 (Mouse IgG2a) with a linear range of 0.6-5 ng/mL (Routinely tested).

Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.

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5e5 of PBMCs were stained with AF555-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HA2H7) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF555 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).