A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).

A double logarithmic fitting curve was plotted for each cytokine (IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ). The present curve of each cytokines was ranging from 9.8 pg/ml to 10000 pg/ml respectively.

The CV of 5 cell culture results with different 3 batches are lower than 20%.（The IL-6 concentrations of cell culture sample II、III are lower than LOQ）

The recovery with 3 different concentrations（2500 pg/ml 、 625 pg/ml 、156.25 pg/ml） spiked into serum, plasma and cell culture are about 80%~120%.