Start by setting the CD45/SSC scatter plot to identify the CD45+ lymphocyte population (A). Within the CD45+ gate, use the CD3/SSC scatter plot to identify the CD3+ T cell population (B). Next, use the CD3/CD4 scatter plot to identify the CD3+CD4+ helper T cells (Th gate) (C), and the CD3/CD8 scatter plot to identify the CD3+CD8+ cytotoxic T cells (Tc gate) (D). For B cells, use the CD3/CD19 scatter plot to identify the CD3-CD19+ population (B gate) (E). Finally, set the CD3/CD16+CD56 scatter plot to assess the CD3-CD56+CD16+ NK cell population (F).