登录 | 注册    关注公众号  
微信公众号
搜索
>产品&服务 > 疱疹病毒疫苗研发解决方案
疱疹病毒疫苗研发解决方案
疱疹病毒种类
VZV

VZV

HSV

HSV

EBV

EBV

HCMV

HCMV

Lipid envelope
Glycoprotein
spikes
Nucleocapsid
Dounle-stranded
DNA genome
Tegument
水痘-带状疱疹病毒(varicella-zoster virus, VZV)是一种有包膜的DNA病毒,是具有高度传染性的儿童常见疾病,在儿童初次感染引起水痘,恢复后病毒潜伏在体内,少数病人在成人后病毒再发而引起带状疱疹。
VZV病毒包括6种糖蛋白,分别为为gE、gB、gH、gI、gC和gL。在受感染的细胞中,其中gE是水痘带状疱疹病毒表达最丰富的蛋白质,在病毒复制和细胞间传导中起重要作用,可作为可识别抗原,因而成为疫苗研究的靶点。

>>> 水痘-带状疱疹病毒(VZV)相关热门产品:gE蛋白

单纯疱疹病毒(Herpes simplex viruses,HSV),属于疱疹病毒科,分为疱疹病毒一型(HSV1)和疱疹病毒二型(HSV2)。HSV是一种广泛分布的嗜神经组织病原体,潜伏期时,单纯疱疹病毒不传染,通过接触病毒复发期皮肤感染区而传染。
HSV是具有囊膜的双链线性DNA病毒,基因组约为152 kb,GC含量高,约占68%。疱疹病毒颗粒的平均直径约为185 nm,核心为双链DNA,其外是由162个衣壳蛋白分子组成的衣壳包裹,间质位于衣壳与囊膜之间,最外层为至少13种病毒糖蛋白组成的双层脂质囊膜结构。HSV在吸附细胞膜表面时,首先是糖蛋白gC和/或gB与细胞表面的硫酸乙酰肝素黏蛋白非特异性结合,从而缩短病毒囊膜与细胞膜的空间距离。随后,gD则与胞膜上的特异性受体结合。这些结合进一步启动gH、gL等,与上述蛋白一起,发挥促进介导病毒囊膜与细胞膜融合的作用。
作为病毒入侵宿主细胞非常关键的包膜糖蛋白gD,与病毒免疫逃脱有关的蛋白gC2和gE2,一方面能产生阻断病毒入侵宿主细胞的抗体,另外一方面能产生防止病毒逃脱免疫系统攻击的抗体,是疫苗设计的重要靶点。

>>> 单纯疱疹病毒(Herpes simplex viruses,HSV)相关热门产品:gH&gL Glycoprotein D (HSV-1) Glycoprotein C (HSV-2) Glycoprotein D (HSV-2) Glycoprotein E (HSV-2)post-gB

EB病毒(Epstein-Barr virus,EBV),是Epstein和Barr在伯基特淋巴瘤细胞系观察到的病毒颗粒,称为Epstein-Barr病毒(EBV),又名人类疱疹病毒4型(HHV-4)。是一种嗜人类淋巴细胞的双链DNA病毒,属于疱疹病毒科,人感染EBV后建立终身潜伏感染,人群感染率超过90%。EBV是一种重要的肿瘤相关病毒,与鼻咽癌、淋巴瘤、胃癌、移植后淋巴增殖症等多种肿瘤的发生密切相关,全世界受EBV相关肿瘤影响的人口达到1%。然而,迄今为止仍没有获得许可的EB病毒预防性疫苗。
EBV通过质膜进入上皮细胞,通过内吞作用进入B细胞。糖蛋白B (gB)是EB病毒进入宿主细胞所必需的主要融合原。gp350/gp220与补体受体2(CR2)或CR1结合启动入侵B细胞,gHgL/gp42与HLA-II结合触发融合前gB。在进入上皮细胞的过程中,BMRF2与整合素结合,然后gHgL与EphA2结合,激活融合前的gB。糖蛋白B(gB)是EBV进入宿主细胞所必需的主要融合原。gH/gL和gB蛋白协同介导EBV融合并进入B细胞和上皮细胞,是极具潜力的EBV疫苗研究靶点。

>>> EB病毒(Epstein-Barr virus,EBV)相关热门产品:gp350 Glycoprotein H & Glycoprotein L (EBV)Glycoprotein B (EBV)

人巨细胞病毒(human cytomegalovirus,HCMV)是一种疱疹病毒科的β属疱疹病毒,其在人群中感染非常普遍,在发达国家成年人HCMV抗体阳性率约为50%,我国则高达60%~80%,原发感染多发生于婴幼儿时期,每年会感染约100万新生儿,这种病毒感染可导致婴儿的终身后遗症,如感觉神经性听力损失和脑损伤。对于免疫功能低下的个体患者,人巨细胞病毒(HCMV)感染也会导致严重的疾病负担。因此,迫切需要开发有效的人巨细胞病毒(HCMV)疫苗来预防感染和与其相关的各种疾病。
HCMV感染可通过不同的入侵机制在多种类型的宿主细胞中引起持续、终生的感染,病毒入侵主要涉及四种包膜糖蛋白复合物:糖蛋白B(gB)寡聚体、gM/gN二聚体、gH/gL/gO三聚体和gH/gL/UL128/UL130/UL131A五聚体复合物(PC)。gB是一种跨膜蛋白,它位于HCMV外壳的表面,作为病毒颗粒与宿主细胞相互作用的重要蛋白,具有广泛的功能。在病毒感染初期,gB可以通过与宿主细胞的表面受体结合,介导病毒进入宿主细胞,由于其能够引起NAb和非NAb应答,已被确定为主要的疫苗靶点之一。gH/gL/gO三聚体可促进所有类型的细胞与gB融合。因此,gH/gL/gO三聚体是HCMV疫苗的潜在靶点,研究表明,gH/gL/gO三聚体可防止HCMV逃逸目前临床测试的疫苗诱导的NAbs。

>>> 人巨细胞病毒(human cytomegalovirus,HCMV)相关热门产品:Glycoprotein B / gBgH&gL&gO:

疱疹病毒疫苗研发解决方案

疱疹病毒疫苗研发解决方案

更多选择:gB,gC,gD,gE,gH&gL,gH&gL&gO等多种疱疹病毒抗原蛋白供您选择;

天然构象:真核HEK293细胞表达,更接近天然构象的蛋白设计;

高均一性:抗原蛋白高均一性经SEC-MALS验证。

精密度高:板内及板间精密度CV值均≤15%;

回收率高:加标回收率在80%-120%内;

提供Human、Mouse不同种属检测用试剂盒,满足临床前及临床阶段的体液免疫评价需求。

灵敏度高:疱疹病毒抗原定量试剂盒灵敏度最高可达78 pg/mL;

检测范围宽,选择灵活:不同检测范围的试剂盒可满足不同样本浓度下的检测需求;

精密度高:板内及板间精密度CV值均≤15%。

高灵敏,高特异;

重组表达,批间稳定性好;

可用于多种疱疹病毒抗原蛋白检测或作为抗体标品。

抗原蛋白

高均一性(SEC-MALS)

VZV
Glycoprotein H & Glycoprotein L/gH & gL (VZV)
Glycoprotein H & Glycoprotein L/gH & gL (VZV)

The purity of Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-V5283) is more than 90% and the molecular weight of this protein is around 105-135 kDa verified by SEC-MALS.

Glycoprotein I & Glycoprotein E/gI & gE (VZV)
Glycoprotein I & Glycoprotein E/gI & gE (VZV)

The purity of Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (Cat. No. GIE-V5283) was greater than 85% as determined by SEC-HPLC.

HSV
Glycoprotein C/gC (HSV)
Glycoprotein C/gC (HSV)

The purity of HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (Cat. No. GLC-V52H3) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

Glycoprotein H & Glycoprotein L/gH & gL (HSV)
Glycoprotein H & Glycoprotein L/gH & gL (HSV)

The purity of HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-H5283) is more than 85% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

EBV
Glycoprotein H & Glycoprotein L/gH & gL (EBV)
Glycoprotein H & Glycoprotein L/gH & gL (EBV)

The purity of Epstein-Barr virus Glycoprotein H&Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag (Cat. No. GHL-E5283) is more than 90% and the molecular weight of this protein is around 100-125kDa verified by SEC-MALS.

Glycoprotein B/gB (EBV)
Glycoprotein B/gB (EBV)

The purity of Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (Cat. No. GLB-E52H3) is more than 85% and the molecular weight of this protein is around 275-305 kDa verified by SEC-MALS.

CMV
Glycoprotein H & Glycoprotein L/gH & gL (HCMV)
Glycoprotein H & Glycoprotein L/gH & gL (HCMV)

The purity of HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) is more than 90% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.

Glycoprotein B/gB (HCMV)
Glycoprotein B/gB (HCMV)

The purity of HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) is more than 95% and the molecular weight of this protein is around 308-376 kDa verified by SEC-MALS.

滑动查看完整产品列表

产品类型 病毒类型 分子 货号 产品描述
抗原蛋白 VZV Glycoprotein H & Glycoprotein L/gH & gL (VZV) GHL-V5283 Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (MALS verified)
Glycoprotein I & Glycoprotein E/gI & gE (VZV) GIE-V5283 Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (HPLC-verified)
Glycoprotein E/gE (VZV) GLE-V52H3 Varicella zoster virus (strain Oka vaccine) Envelope Glycoprotein E (gE), His Tag (MALS verified)
HSV Glycoprotein H & Glycoprotein L/gH & gL (HSV) GHL-H5283 HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (MALS verified)
Glycoprotein B/gB (HSV) GLB-H52H3 HSV-2 (strain 333) Glycoprotein B (gB), His Tag (MALS verified)
Glycoprotein C/gC (HSV) GLC-V52H3 HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified)
Glycoprotein D/gD (HSV) GLD-V52H3 HSV-1 (strain 17) Envelope Glycoprotein D (gD), His Tag (MALS verified)
Glycoprotein D/gD (HSV) GLD-V52H4 HSV-2 (strain 333) Envelope Glycoprotein D (gD), His Tag (MALS verified)
Glycoprotein E/gE (HSV) GLE-H52H4 HSV-2 (HG52) Glycoprotein E Protein, His Tag (MALS verified)
Glycoprotein B/gB (HSV) POB-V52H3 HSV-1 (strain KOS) post-gB Protein, His Tag (MALS verified)
EBV Glycoprotein H & Glycoprotein L/gH & gL (EBV) GHL-E5283 Epstein-Barr virus Glycoprotein H&Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag (MALS verified)
Glycoprotein B/gB (EBV) GLB-E52H3 Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (MALS verified)
CMV Glycoprotein B/gB (HCMV) CMB-V5255 HCMV Glycoprotein B (gB) Protein, Fc Tag
Glycoprotein B/gB (HCMV) CMB-V52H4 HCMV (strain AD169) Glycoprotein B (gB), His Tag (MALS verified)
Glycoprotein B/gB (HCMV) CMB-V52H5 HCMV (strain Merlin) Glycoprotein B (gB), His Tag (MALS verified)
Glycoprotein H & Glycoprotein L/gH & gL (HCMV) GHL-H5243 HHV-5 (strain AD169) Glycoprotein H&L, His Tag (MALS verified)
Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) GLO-H5283 HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag
Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) GLO-V5243 Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (MALS verified)
抗体

高活性(ELISA/SPR)

Anti-Glycoprotein E VZV Antibody (SPR)
RSF-V52H6

Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (1B10) (Cat. No. VZV-M544) captured on Protein A Chip can bind Varicella zoster virus (strain Oka vaccine) Envelope Glycoprotein E (gE), His Tag (Cat. No. GLE-V52H3) with an affinity constant of 5.63 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

Anti-gC-HSV-2 (strain 333) Antibody (ELISA)
RSF-V52H6

Immobilized HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) (Cat. No. GLC-V52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) (Cat. No. HSV-Y184) with a linear range of 0.098-6.25 ng/mL (QC tested).

产品类型 病毒类型 分子 货号 产品描述
抗体 VZV Glycoprotein E/gE (VZV) VZV-BLM545 Biotinylated Monoclonal Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (4G2)
Glycoprotein E/gE (VZV) VZV-M544 Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (1B10) (MALS verified)
HSV Glycoprotein D/gD (HSV) GLD-M609 Monoclonal Anti-HSV-2-Glycoprotein D Antibody, Human IgG1 (3H9)
Glycoprotein D/gD (HSV) GLD-Y111 Monoclonal Glycoprotein D (HSV-2) Antibody, Mouse IgG1 (3H9)
Glycoprotein C/gC (HSV) HSV-Y184 Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12)
试剂盒
VZV
Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit
Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit

Detection of Monoclonal Glycoprotein E (VZV) titer by sandwich-ELISA Assay.
Immobilized Anti-Glycoprotein E (VZV) Antibody can bind Glycoprotein E (VZV). Detection was performed using Biotin-Anti-Glycoprotein E (VZV) Antibody with sensitivity of 78 pg/mL (QC tested).

Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)
Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

HSV
ELISA- Anti-gC-HSV-2 (strain 333) Antibody Pair
ELISA- Anti-gC-HSV-2 (strain 333) Antibody Pair

The specific Anti-gC-HSV-2 (strain 333) Antibody pair (Clone:9A12/3A12) for the detection of HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) (Cat. No. GLC-V52H3), linear range of 1.56-0.024ng/mL. (sandwich ELISA)

Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)
Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

产品类型 病毒类型 分子 货号 产品描述
抗原定量试剂盒 VZV Glycoprotein E/gE (VZV) RAS-A102 Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit
Glycoprotein E/gE (VZV) RAS-A185 Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit (For Vaccine Development)
抗体检测试剂盒 VZV Glycoprotein E/gE (VZV) RAS-T103 Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)
Glycoprotein E/gE (VZV) RAS-T132 Human Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)
HSV Glycoprotein C/gC (HSV) RAS-T197 Mouse Anti-HSV-2 (strain 333) Antibody IgG Titer ELISA Assay Kit (gC)
Glycoprotein D/gD (HSV) RAS-T198 Mouse Anti-HSV-2 (strain 333) Antibody IgG Titer ELISA Assay Kit (gD)
常见FAQ

对于HSV众多的抗原蛋白(gH&gL、gC、gD、gE、gB等),哪个最适合作为候选疫苗的靶点?

gB是疱疹病毒中最保守的膜融合糖蛋白,具有驱动病毒包膜和宿主细胞膜融合的作用;gC、gB协同作用帮助宿主细胞表面浓缩病毒颗粒;gD可以与宿主细胞特异性结合;gH&gL 异二聚体可以发挥信号传递作用,从而促进病毒包膜和宿主细胞膜融合。在选择候选疫苗靶点的过程中,可以根据自己的研究方向以及抗原蛋白的特点进行选择。

如何确保试剂盒检测结果的稳定性?

在售试剂盒产品均参考【9012生物样品定量分析方法验证指导原则】对试剂盒进行方法学验证,以确保检测结果的稳定性。

对于抗原定量检测试剂盒,能否使用自己的标准品替换试剂盒中标准品进行定量检测?

可以进行替换,但需要您使用自己的标准品重新进行方法学验证和定量范围的摸索,若您需要方法学支持,可点击右下角在线咨询联系我们。

同样是VZV gE蛋白抗原定量检测试剂盒,RAS-A185和RAS-A102的区别是什么?

RAS-A185和RAS-A102两款产品的灵敏度和显色方式有差异,RAS-A185为HRP-Anti-Glycoprotein E (VZV) Antibody直接显色,RAS-A102为Biotin-Anti-Glycoprotein E (VZV) Antibody+Streptavidin-HRP显色,二者都可以用于VZV gE抗原蛋白的定量检测。RAS-A102的灵敏度更高,可根据蛋白的表达量选择合适的kit。

ACRO质量管理体系

消息提示

请输入您的联系方式,再点击提交!

确定