快速扩增,支持大规模培养!
化学成分限定 无动物源 无需血清或血替添加 更快扩增倍率
T淋巴细胞在人体免疫系统中发挥着至关重要的作用,利用特定T细胞的固有功能来对抗疾病已成为突破性的技术手段。T细胞培养基提供T细胞增殖所需的营养基质,通过模拟体内微环境,使分离后的T细胞在体外能够正常扩增,同时保持其结构和功能。T细胞培养基的发展经历了三个重要阶段,其中化学成分明确的培养基因其能够确保批次间一致性、可追溯性和产品稳定性,成为未来的主流选择。
ACROBiosystems百普赛斯CelThera™ GMP级T细胞培养基——采用创新的化学限定配方,不含血清和动物源成分,专注于提升T细胞培养的稳定性与可控性,助力增强T细胞增殖与活力,以提升整体生产效率。
化学成分限定、无动物源、高批间一致性;
无需血清或血替添加;
更高更快扩增倍率(7天扩增100倍,13天扩增3000倍),且可维持良好的细胞活率;
达到理想的CD4+和CD8+T细胞比例(1:1)的时间更短;
更快促进细胞中TN/SCM比例增加。
促进T细胞、CAR-T细胞、TCR-T、外周血淋巴细胞(PBL)等T源细胞的快速扩增;
支持从临床前开发到生产的规模放大;
遵循GMP生产管理体系;
提供完整申报支持文档、全套方法学验证报告等文件,已完成FDA DMF备案。
CelThera™ GMP级T细胞培养基以卓越的无血清配方,结合细胞因子的完美支持,助力高效T细胞扩增,满足临床及商业化规模的严格要求。我们通过在多种规模化体系下的全面验证,包括6孔板、T225培养瓶和3L体系,展现了其在不同生产场景中的卓越适用性,为全球T细胞疗法的商业化生产提供了科学依据和强有力的技术支持。
Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) or T cell culture medium (Competitor L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACROBiosystems) can be comparable with the Competitor L +2.5% SR. Notably, the cells exhibits better expansion in CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101).
Human PBMCs were cultured with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) or T cell culture medium (Competitor L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACROBiosystems) can be comparable with the Competitor L +2.5% SR.
Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can promote the expansion of these cells with a reasonable cell viability.
Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can increase the percentage of the CD3+ cells. With the post-culture time, the proportion of CD4 cells gradually decreased, and the proportion of CD8 cells gradually increased.
Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), cultured with CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The results showed that GMP human IL-2 protein, GMP monoclonal anti-human CD3 antibody (OKT3), GMP monoclonal anti-human CD28 antibody and CelThrea™ GMP T cell expansion medium could be used to culture T cells in a 3L large system. It can efficiently expand cells with high viability.
Cell ratio of the human various T cells. Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), and cultured in CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The result shows that percentage of CD3+ cells in living cells is above 99%. At the end of the culture process, the majority memory phenotype of T cells are TN/SCM, followed by TCM, which means that the cell harvest will have a promising effect, such as sustained proliferation, prolonged survival, and so on.
GMP-CM3101-1是CelThera™ GMP T Cell Expansion 的补充剂,不含血清或血替。
CelThera™ 初始培养基中未添加任何因子、抗体成分,使用时需单独配置添加。
两个组分混合后可以于4℃保存1个月,但是建议混合后尽量2周内使用完毕。
使用时,在室温环境化冻GMP-CM3101-1,随后将全部GMP-CM3101-1液体转移至GMP-CM3101中,充分混匀后再使用。
ACROBiosystems百普赛斯生物科技股份有限公司
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