CelThera™GMP级别T细胞培养基

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can promote the expansion of these cells with a reasonable cell viability.

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can increase the percentage of the CD3+ cells. With the post-culture time, the proportion of CD4 cells gradually decreased, and the proportion of CD8 cells gradually increased.

Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), cultured with CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The results showed that GMP human IL-2 protein, GMP monoclonal anti-human CD3 antibody (OKT3), GMP monoclonal anti-human CD28 antibody and CelThrea™ GMP T cell expansion medium could be used to culture T cells in a 3L large system. It can efficiently expand cells with high viability.

Cell ratio of the human various T cells. Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), and cultured in CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The result shows that percentage of CD3+ cells in living cells is above 99%. At the end of the culture process, the majority memory phenotype of T cells are TN/SCM, followed by TCM, which means that the cell harvest will have a promising effect, such as sustained proliferation, prolonged survival, and so on.