过表达及报告基因细胞株-ADC 药物筛选以及功能评价

FACS analysis of Nectin-4 on HEK293/Human Nectin-4 Stable Cell Line.
FACS assay shows that Anti-Nectin-4 antibody can bind to HEK293/Human Nectin-4 Stable Cell Line (Cat. No. CHEK-ATP035). HEK293/Human Nectin-4 stable cells was red line, Negative control HEK293 cells was grey line (QC tested).

FACS assay shows that Anti-TROP-2 antibody can bind to HEK293/Human TROP-2 Stable Cell Line.
HEK293/Human TROP-2 stable cells (Cat. No. CHEK-ATP036) was red line, Negative control HEK293 cells was grey line (QC tested).
 

ADCC response to anti-human CD20 antibody (RLU). Anti-human CD20 antibody-induced ADCC activity was evaluated using Human CD16a (158V) (Luc) Jurkat Reporter Cell in the presence of Raji cells that express CD20 endogenously. The EC50 of anti-human CD20 antibody was approximately 0.0028 μg/mL.

ADCC response to anti-human CD20 antibody (FOLD). Anti-human CD20 antibody-induced ADCC activity was evaluated using Human CD16a (158V) (Luc) Jurkat Reporter Cell in the presence of Raji cells that express CD20 endogenously. The max induction fold was approximately 854.

ADCP response to anti-human CD20 antibman CD20 antibody-induced ADCP activity was evaluated using Human CD32a (131H) (Luc) Jurkat Reody (RLU). Anti-huporter Cell in the presence of Raji cells that express CD20 endogenously. The EC50 was approximately 0.1054 μg/mL.

ADCP response to anti-human CD20 antibody (FOLD). Anti-human CD20 antibody-induced ADCP activity was evaluated using Human CD32a (131H) (Luc) Jurkat Reporter Cell in the presence of Raji cells that express CD20 endogenously. The max induction fold was approximately 277.90.

Human CD16a (158V) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF067)

Expression analysis of human CD16a (158V) on Human CD16a (158V) (Luc) Jurkat Reporter Cell by FACS. Human CD16a (158V) (Luc) Jurkat Reporter Cell or negative control cell were stained with PE-labeled anti-human CD16a antibody.

Human CD32a (131H) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF069)

Expression analysis of human CD32a on Human CD32a (131H) (Luc) Jurkat Reporter Cell by FACS. Cell surface staining was performed on Human CD32a (131H) (Luc) Jurkat Reporter Cell or negative control cell using PE-labeled anti-human CD32a antibody.

Human CD16a (158V) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF067)

Passage stability analysis by Signaling Bioassay. The continuously growing Human CD16a (158V) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of anti-human CD20 antibody in the presence of Raji cells that express CD20 endogenously. Anti-human CD20 antibody stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 14-26.

Human CD32a (131H) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF069)

Passage stability analysis by Signaling Bioassay. The continuously growing Human CD32a (131H) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of Anti-human CD20 antibody in the presence of Raji cells. Anti-human CD20 antibody stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-23.