免疫检查点(immune checkpoint)是免疫系统功能的关键调节因子。针对免疫检查点开发抗体药物用于癌症、肿瘤、自身免疫病等疾病的治疗是免疫疗法的重要手段。CTLA-4和PD-1/PD-L1是目前研究广泛的抑制性免疫检查点途径,通过阻断免疫检查点来治疗恶性肿瘤的研究已经扩展到对LAG-3、TIM-3、TIGIT、VISTA、B7-H3等免疫共抑制分子的抗体药开发,同时针对OX40、ICOS、GITR、4-1BB、CD40等免疫共刺激分子靶向药物也处于研发阶段。因此,如何高效开展免疫检查点分子抗体药物的研发仍是目前的关注重点。
点击分子名查看详情
分子 | 种属 | 标签 | 标记 |
验证数据 ( 可提供 Protocol ) |
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PD-1 | Human, Mouse, Canine, Cynomolgus, Rabbit, Rat, Rhesus macaque | Fc, His, Fc & His, Fc & Avi & His, Fc & Avi, His & Avi, mFc& Avi, Twin-Strep, Llama Fc , mFc | Biotin-labeled, PE-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, BLI, SPR, |
PD-L1 | Human, Mouse, Rabbit, Canine, Cynomolgus / Rhesus macaque | Fc, His, Fc & Avi, His & Avi, Fc & Avi & His, mFc, LlamaFc, mFc& Avi, Twin-Strep | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, SPR, |
PD-L2 | Human, Mouse | Fc, His, Fc & Avi, Avi & His, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, HPLC, ELISA, SPR, BLI,, Cell based assay |
B7-H3 | Human, Mouse, Cynomolgus | Fc & Avi, Avi & His, His, Fc, Avi & His, , | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, Cell based assay |
B7-H4 | Human, Mouse | His, Fc | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA |
B7-H5 | Human, Mouse, Rhesus macaque | Fc & Avi, Avi & His, His, Fc, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, SPR, BLI |
BTLA | Human, Mouse | Fc & Avi, His & Avi, His, mFc, Fc | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA |
CD80(B7-1) | Human, Mouse, Cynomolgus / Rhesus macaque | Fc, Fc & Avi, Avi & His, mFc, His | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, |
CD86(B7-2) | Human, Mouse, Cynomolgus / Rhesus macaque | Fc & Avi, Fc, His | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, BLI, |
CD96 | Human | His & Avi, His, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, |
CD47 | Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine | Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc | Biotin-labeled, FITC-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, SPR, BLI, |
CD155 | Human, Mouse, Rhesus macaque | Fc & Avi, His & Avi, mFc, Fc, His | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, |
CTLA-4 | Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque | Fc, His, Fc & Avi, His & Avi, mFc, mFc& Avi | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, SPR, BLI, |
LAG-3 | Human, Mouse, Rat, Cynomolgus, Marmoset | Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, SPR, BLI, |
TIGIT | Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine | mFc& Avi, Fc & Avi, His & Avi, His, Fc, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI |
TIM-3 | Human, Mouse, Cynomolgus | Avi & His, mFc, Fc, His | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, SPR |
CD111 | Human | His, Fc | Unconjugated | SDS-PAGE, ELISA, BLI |
DNAM-1 | Human, Cynomolgus | Fc & Avi, His, Fc | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA, BLI, |
Galectin-9 | Human | His, MBP & His | Unconjugated | SDS-PAGE |
HVEM | Human | His, Twin-Strep, Fc, mFc, Fc & Avi | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA, BLI |
Nectin-2 | Human | His, Fc | Biotin-labeled, Unconjugated | SDS-PAGE, HPLC, ELISA, BLI |
Nectin-3 | Human | His, Fc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI |
PVRIG | Human, Mouse, Cynomolgus | Fc & Avi, mFc& Avi, His & Avi, His, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI |
SIRP alpha | Human, Mouse, Cynomolgus | Fc, His & Avi, mFc& Avi, mFc, His | Biotin-labeled, PE-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, BLI, |
SIRP alpha V2 | Human | His | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA |
SIRP gamma | Human | Fc | Unconjugated | SDS-PAGE, ELISA |
CD160 | Human | His | Unconjugated | ELISA |
分子 | 种属 | 标签 | 标记 |
验证数据 ( 可提供 Protocol ) |
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4-1BB Ligand | Human, Mouse, Rat, Cynomolgus | Fc, Fc & Avi, mFc, His & Flag | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, Cell based assay |
4-1BB | Human, Mouse, Rat, Rabbit, Cynomolgus / Rhesus macaque, Canine | His, Fc, Fc & Avi, His & Avi, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, SPR |
OX40 Ligand | Human, Mouse, Rhesus macaque | Fc, His, His & Avi, Avi & Fc, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, |
OX40 | Human, Mouse, Rabbit, Rat, Rhesus macaque, Marmoset | His, Fc, His & Avi, Fc & Avi, Avi & His, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, |
ICOS Ligand (B7-H2) |
Human, Mouse, Cynomolgus | His, Fc | Unconjugated | SDS-PAGE, ELISA |
ICOS | Human, Mouse, Cynomolgus | His, Fc, His & Avi | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA |
GITR Ligand | Human, Mouse | His, Fc, Avi & Fc | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA |
GITR | Human, Mouse, Rat, Rhesus macaque, Canine | His, Fc, His & Avi, Fc & Avi, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA |
CD48 | Human | His, Fc, mFc | Unconjugated | SDS-PAGE, ELISA |
HVEM | Human | His, Fc, Fc & Avi, mFc, TwiStrep | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, BLI |
Nectin-2 | Human | His, Fc, Fc & Avi, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, BLI |
CD40 Ligand | Human, Mouse, Human / Rhesus macaque | His, Fc, His & Avi, Avi & Fc, Avi & His & Flag, His & Flag, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI |
CD40 | Human, Mouse, Rabbit, Cynomolgus, Rhesus macaque | His, Fc, Avi & His, Fc & Avi, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, |
CD30 ligand | Human | His, mFc | Unconjugated | SDS-PAGE, ELISA, BLI, |
CD30 | Human | His, Fc, Avi & His, LlamaFc | Biotin-labeled, PE-labeled, FITC-labeled, Unconjugated | SDS-PAGE, MALS, ELISA, BLI, |
CD28 | Mouse, Human / Cynomolgus / Rhesus macaque | His, Fc, His & Avi, Fc & Avi, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, HPLC, ELISA, SPR, BLI |
CD27 Ligand | Human, Mouse | His & Avi, mFc, His, His & Avi & Flag, His & Flag, Fc | Biotin-labeled, FITC-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA |
CD27 | Human, Mouse | His, Fc, Fc & Avi, mFc | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA |
B7-1 | Human, Mouse, Cynomolgus / Rhesus macaque | Fc, Fc & Avi, Avi & His, mFc, His | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, |
B7-2 | Human, Mouse, Cynomolgus / Rhesus macaque | His, Fc, Fc & Avi | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, HPLC, ELISA, BLI, |
LIGHT | Human | His, mFc, Fc | Unconjugated | SDS-PAGE, MALS, ELISA, BLI |
B7-H7 | Human, Cynomolgus | His, Fc, His & Avi, Fc & Avi | Biotin-labeled, Unconjugated | SDS-PAGE, MALS, BLI |
B7-H6 | Human, Cynomolgus | His, His & Avi, Fc & Avi | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA, SPR |
2B4 | Human | His, Fc, Avi & His | Biotin-labeled, Unconjugated | SDS-PAGE, ELISA |
CD28H | Human | His, Fc | Unconjugated | SDS-PAGE, ELISA |
NKp30 | Human | His, Fc | Unconjugated | SDS-PAGE, SPR |
LILRA&B | LILRA1 | LILRA2 | LILRA3 | LILRA5 | LILRA6 |
LILRB1 | LILRB2 | LILRB3 | LILRB4 | LILRB5 | |
LILRA&2 | LAIR-1 | LAIR-2 | |||
SLAMF | SLAMF1 | SLAMF2(CD48) | SLAMF3(CD229) | SLAMF4(2B4) | SLAMF5(CD84) |
SLAMF6(NTB-A) | SLAMF7 | SLAMF8(BLAME) |
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Butyrophilins | BTN1A1 | BTN3A1 | BTN3A2 | BTN3A3 |
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更多 | CD161 | MARCO | MSP1D1 | Nectin-1 | Nectin-4 |
TREM2 | ILDR2 |
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货号 | 产品详情 | 检测方法 | 订购/预购 |
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EP-143 | TNF-alpha [Biotinylated] : TNFR1 Inhibitor Screening ELISA Kit | 显色反应 | |
EP-147 | TNF-alpha [Biotinylated] : TNFR2 Inhibitor Screening ELISA Kit | 显色反应 | |
EP-116 | B7-1 [Biotinylated] : CTLA-4 Inhibitor Screening ELISA Kit | 显色反应 | |
EP-117 | B7-2 [Biotinylated] : CTLA-4 Inhibitor Screening ELISA Kit | 显色反应 | |
EP-126 | CTLA-4 [Biotinylated] : B7-2 Inhibitor Screening ELISA Kit | 显色反应 | |
EP-120 | PD-1 [Biotinylated] : PD-L2 Inhibitor Screening ELISA Kit | 显色反应 | |
EP-130 | PD-1 : PD-L1 [Biotinylated] Inhibitor Screening ELISA Kit | 显色反应 | |
EP-133 | PD-L1 : PD-1 [Biotinylated] Inhibitor Screening ELISA Kit | 显色反应 | |
EP-138 | CD47 [Biotinylated] : SIRP alpha Inhibitor Screening ELISA Kit | 显色反应 | |
EP-135 | CD30 [Biotinylated] : CD30L Inhibitor Screening ELISA Kit | 显色反应 | |
EP-101 | PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Kit | 显色反应 |
可免费提供内部实验操作方法以供参考,进入产品详情页即可申请相关Protocol
重组蛋白的均一性及聚集状态是否与天然状态保持一致是抗体药关注的重点,高纯度高均一性的产品是免疫以及抗体筛选的必要条件。
The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) is more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.
Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)(Cat. No. PD1-H82E4)
PD-1&PD-L1通路
Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221 ) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.
Protocol
PD-1&PD-L1通路
Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).
Protocol
此外,我们还开发了PD1: PD-L1(Cat. No. EP-101)和CD47: SIRP alpha(Cat. No. EP-102)抑制剂筛选测定试剂盒,可以快速并且高通量地筛选抑制性抗体或小分子。
LAG-3通路
Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
LAG-3通路
Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
TIGIT通路
Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6 ) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).
Protocol
CD47&SIPR α通路(细胞水平验证)
FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL
Protocol
4-1BB通路
Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.20-0.44 μg/mL (Routinely tested).
Protocol
CTLA-4通路
Immobilized Human CTLA-4, His Tag, active dimer (Cat. No.CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag,His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).
Protocol