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 >  Protein>IL-6 >IL6-H4218

Human IL-6 Protein, premium grade

GMP version GMP-L06H27 is now available for seamless transition.

分子别名(Synonym)

IL6,Interleukin-6,BSF2,HSF,IFNB2

表达区间及表达系统(Source)

Human IL-6, premium grade (IL6-H4218) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000591.1).

Predicted N-terminus: Val 30

Human IL-6, premium grade (IL6-H4218), designed for preclinical stage, has the same activity and performance with GMP Human IL-6 (GMP-L06H27), which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

Request for sequence

蛋白结构(Molecular Characterization)

IL-6 Structure

This protein carries no "tag".

The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

宿主蛋白残留(Host Cell Protein)

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留(Host Cell DNA)

<0.02 ng/μg of protein tested by qPCR.

无菌(Sterility)

Negative

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-6 SDS-PAGE

Human IL-6, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-Bioactivity CELL BASE

IL-6 CELL

Human IL-6, premium grade (Cat. No. IL6-H4218) stimulates proliferation of TF-1 human erythroleukemic cell line. The specific activity of Human IL-6, premium grade is > 1.00×10^8 IU/mg, which is calibrated against human IL-6 WHO International Standard (NIBSC code: 21/308) (QC tested).

Protocol

IL-6 BATCH CELL BASE

Activity of three different production batches of Human IL-6 Protein, premium grade (Cat. No. IL6-H4218).

IL-6 CELL

The activity of Human IL-6 Protein, premium grade (Cat. No. IL6-H4218) was higher than other competing products.

 

稳定性(Stability)

IL-6 STABILITY

The Cell based assay shows that Human IL-6 Protein, premium grade (Cat. No. IL6-H4218) is stable at 37°C for 48 hours.

IL-6 STABILITY

The Cell based assay shows that Human IL-6 Protein, premium grade (Cat. No. IL6-H4218) is stable after freezing and thawing 3 times.

IL-6 STABILITY

The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-6.

 

背景(Background)

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

文献引用(Citations)

 

前沿进展

 
 
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IL-6靶点信息
英文全称:Interleukin-6
中文全称:白细胞介素-6
种类:Homo sapiens
上市药物数量:6详情
临床药物数量:18详情
最高研发阶段:批准上市
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