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 >  Protein>BAFFR >BAR-H5257

Human BAFFR / TNFRSF13C Protein, Fc Tag

分子别名(Synonym)

BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R

表达区间及表达系统(Source)

Human BAFFR, Fc Tag (BAR-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).

Predicted N-terminus: Ser 7

Request for sequence

蛋白结构(Molecular Characterization)

BAFFR Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 33.2 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

BAFFR SDS-PAGE

Human BAFFR, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

BAFFR ELISA

Immobilized Human BAFF, His Tag at 5 μg/mL (100 μL/well) can bind Human BAFFR, Fc Tag (Cat. No. BAR-H5257) with a linear range of 2-31 ng/mL (QC tested).

Protocol

BAFFR BATCH ELISA
BAFFR ELISA

Immobilized Human BAFFR, Fc Tag (Cat. No. BAR-H5257) at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

BAFFR ELISA

Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag (Cat. No. BAR-H5257): Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) (Cat. No. BAF-H82Q2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.7363 μg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

BAFFR SPR

Immobilized Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) (Cat. No. BAF-H82F3) on SA Chip can bind Human BAFFR, Fc Tag (Cat. No. BAR-H5257) with an affinity constant of 26.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

 

前沿进展

 
 
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BAFFR靶点信息
英文全称:B-cell activating factor receptor
中文全称:B细胞活化因子受体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床三期
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