膜杰作
Star Staining
分子别名（Synonym）
IL4,BCGF1,BSF1
表达区间及表达系统（Source）
Biotinylated Human IL-4, Avitag,His Tag (IL4-H82E0) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # AAH67514).
Predicted N-terminus: His 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag
The protein has a calculated MW of 17.6 kDa. The protein migrates as 22 kDa under reducing (R) condition, and 21 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IL-4, Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) is more than 85% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) with a linear range of 2-16 ng/mL (QC tested).
Protocol
Immobilized Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221): Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) binding reactions. The half maximal inhibitory concentration (IC50) is 0.505 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-Bioactivity CELL BASE
Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.19-2.64 ng/mL (Routinely tested).
Protocol
背景（Background）
Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.
