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 >  Protein>Fc gamma RIII / CD16 >FC6-C82E0

Biotinylated Cynomolgus Fc gamma RIII / CD16 Protein, His,Avitag™ (MALS & BLI verified)

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分子别名(Synonym)

FCGR3

表达区间及表达系统(Source)

Biotinylated Cynomolgus CD16, His,Avitag (FC6-C82E0) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # Q8SPW2-1 ).

Predicted N-terminus: Gly 17

Request for sequence

蛋白结构(Molecular Characterization)

Fc gamma RIII / CD16 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 25.7 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Fc gamma RIII / CD16 SDS-PAGE

Biotinylated Cynomolgus CD16, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Fc gamma RIII / CD16 SEC-MALS

The purity of Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) is more than 90% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Fc gamma RIII / CD16 ELISA

Immobilized Rituximab at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) with a linear range of 0.039-1.25 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

Fc gamma RIII / CD16 SPR

Captured Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) on Biotin CAP - Series S sensor Chip can bind MabThera® (Rituximab) with an affinity constant of 0.63 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Fc gamma RIII / CD16 BLI

Loaded Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) on SA Biosensor, can bind Rituximab with an affinity constant of 0.401 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.
CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

 

前沿进展

 
 
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Fc gamma RIII / CD16靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor III-A
中文全称:低亲和力免疫球蛋白γFc段受体III-A
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床二期
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