膜杰作
Star Staining
分子别名（Synonym）
TSLP
表达区间及表达系统（Source）
Biotinylated Human TSLP, His,Avitag (TSP-H82Eb) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).
Predicted N-terminus: Tyr 29
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 18.6 kDa. The protein migrates as 22-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Biotinylated Human TSLP, His,Avitag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) is more than 85% and the molecular weight of this protein is around 23-33 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).
Protocol
Immobilized Anti-TSLP MAb, Human IgG2 at 2 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) on SA Biosensor, can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with an affinity constant of 15.2 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with an affinity constant of 13.2 nM as determined in BLI assay (ForteBio Octet R8)(Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Human TSLP R (Luc) HEK293 Reporter Cells were stained with 100 μL of 1 μg/mL of Biotinylated Human TSLP Protein, His,Avitag (Cat. No. TSP-H82Eb) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
+添加评论
- 151XXXXXXX5
- 我们应用于于抗体筛选，经常购买贵公司acro的产品，每个月基本上都会固定采买，主要应用于Elisa分子水平的检测，总之贵公司的产品很不错，是筛选与Elisa的首选蛋白，还会经常回购，涉及公司保密问题，图就不放啦
- 2022-12-6
- 181XXXXXXX6
- 我们购买这款蛋白用于筛选抗体，主要用在ELISA实验，这款蛋白灵敏度高，蛋白活性很好，实验取得了很好的数据，一直在Acro购买蛋白，Acro值得信赖。
- 2023-10-30
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背景（Background）
Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
