膜杰作
Star Staining
分子别名（Synonym）
TNFRSF17,CD269,BCM,BCMA
表达区间及表达系统（Source）
Biotinylated Human BCMA, His,Avitag, premium grade (BCA-H82E4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
Predicted N-terminus: Met 1
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 9.6 kDa. The protein migrates as 15-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human BCMA, His,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Biotinylated Human BCMA, His,Avitag, premium grade (Cat. No. BCA-H82E4) was greater than 85% as determined by SEC-HPLC.
活性（Bioactivity）-FACS
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human BCMA, His,Avitag, premium grade (Cat. No. BCA-H82E4) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His,Avitag, premium grade (Cat. No. BCA-H82E4) with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
Immobilized Anti-Human BCMA MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His,Avitag, premium grade (Cat. No. BCA-H82E4) with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
Immobilized Human APRIL, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His,Avitag, premium grade (Cat. No. BCA-H82E4) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) on Protein A Biosensor, can bind Biotinylated Human BCMA, His,Avitag, premium grade (Cat. No. BCA-H82E4) with an affinity constant of 0.55 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
