膜杰作
Star Staining
分子别名（Synonym）
TSLP R,CRLF2,IL-XR,ILXR,p2RY8,CRLF2 fusion,TSLP receptor,TSLPR,CRL2,CRLF2Y,Cytokine receptor-like 2
表达区间及表达系统（Source）
Human TSLP R, Fc Tag (TSR-H525a) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Lys 231 (Accession # Q9HC73-1).
Predicted N-terminus: Gly 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.4 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Human TSLP R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TSLP R, Fc Tag (Cat. No. TSR-H525a) is more than 90% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).
Protocol
Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. TSP-H82E0) immobilized at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Captured Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 1.81 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Captured Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 4.22 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with an affinity constant of 16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with an affinity constant of 24.7 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) with an affinity constant of 23 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with an affinity constant of 13.2 nM as determined in BLI assay (ForteBio Octet R8)(Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag (Cat. No. TSP-H82E0) with an affinity constant of 9.67 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
+添加评论
- 182XXXXXXX6
- 首次用到TSLP R重组蛋白，首先还是ACRO，主要用于抗体活性检测。蛋白活性经得起验证，在多次重复实验中，所得到的数据结果都高度一致，这让我们对后续实验的开展充满了信心，也使得我们的研究成果更具可信度，支持ACRO。
- 2025-2-20
- 199XXXXXXX5
- 使用过多款ACRO的TSLP蛋白了，人猴不同种属的蛋白质量都很nice，本次使用TSLP R蛋白主要用于单克隆抗体BLI活性检测，蛋白活性很高，用先固化蛋白再与抗体结合的方式检测的，数据拟合结果完全符合预期，值得点赞。
- 2025-2-10
产品推荐（Recommended Products）
背景（Background）
Receptor for thymic stromal lymphopoietin (TSLP). CRLF2 / TSLPR is a member of the type I cytokine receptor family. Together with the interleukin 7 receptor (IL7R) and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system.
