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Human TSLP Protein, His Tag, premium grade

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分子别名(Synonym)

TSLP

表达区间及表达系统(Source)

Human TSLP Protein, His Tag, premium grade (TSP-H52Hb) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).

Predicted N-terminus: Tyr 29

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

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蛋白结构(Molecular Characterization)

TSLP Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.8 kDa. The protein migrates as 20-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

无菌(Sterility)

Negative

支原体(Mycoplasma)

Negative.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 24 months under sterile conditions after reconstitution.

质量管理控制体系(QMS)

  1. 质量管理体系(ISO, GMP)
  2. 质量优势
  3. 质控流程
 

电泳(SDS-PAGE)

TSLP SDS-PAGE

Human TSLP Protein, His Tag, premium grade on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-Bioactivity CELL BASE

TSLP CELL

Response to human TSLP protein (Fold).
The TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Human TSLP Protein, His Tag, premium grade (AcroBiosystems, Cat. No. TSP-H52Hb). The max induction fold was approximately 45.15 (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

TSLP FACS

2e5 of Human TSLP R (Luc) HEK293 Reporter Cells were stained with 100 μL of 1 μg/mL of Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) and negative control protein respectively, washed and then followed by PE anti-His Tag Antibody and analyzed with FACS (Routinely tested).

Protocol

 

活性(Bioactivity)-ELISA

TSLP ELISA

Immobilized Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 0.2-4 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

TSLP SPR

Captured Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 1.81 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

TSLP SPR

Monoclonal Anti-Human TSLP Antibody (Human IgG2) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with an affinity constant of 5.47 pM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

TSLP BLI

Loaded Monoclonal Anti-Human TSLP Antibody, Human IgG2 on AHC Biosensor, can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with an affinity constant of 0.263 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

TSLP BLI

Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with an affinity constant of 24.7 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).

Protocol

 
 
ACRO质量管理体系
 
 

背景(Background)

Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cD encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.

 

前沿进展

Ultraviolet-treated riboflavin alleviates atopic dermatitis by inhibiting NLRP3 inflammasome activation and M1 macrophage polarization via histone lactylation
Ge, Qiu, Liu et al
Biochem Pharmacol (2025) 236, 116879
Abstract: Atopic dermatitis (AD) is a chronic inflammatory skin disorder requiring improved therapeutic strategies. This study investigates the potential of ultraviolet (UV)-treated riboflavin in AD treatment. Using a MC903-induced mouse model, we demonstrate that topical UV-treated riboflavin significantly attenuates AD progression. Mechanistically, UV-treated riboflavin suppresses macrophage nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome activation by reducing histone H3 lysine 9 lactylation (H3K9la) on NLRP3 and apoptosis-associated speck-like protein containing a CARD (ASC) promoter, decreasing interleukin-1β (IL-1β) secretion and subsequent keratinocyte-derived thymic stromal lymphopoietin (TSLP) production. It also directly inhibits inflammatory cytokine expression in keratinocytes. NLRP3 activation in vivo partially reverses these effects, confirming the central role of NLRP3 inflammasome inhibition. Our findings reveal a novel epigenetic mechanism of UV-treated riboflavin in modulating immune responses in AD, highlighting its potential as a therapeutic strategy for inflammatory skin disorders.Copyright © 2025 The Authors. Published by Elsevier Inc. All rights reserved.
IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis
Tani-Ichi, Abe, Miyachi et al
J Immunol (2025)
Abstract: Regulatory T cells (Tregs) mediate tissue homeostasis and repair. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs. Here, we show that IL-33R (ST2)-expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Treg-specific IL-7Rα-deficient mice exhibited reduced adipose ST2+ Tregs and impaired glucose tolerance, whereas IL-7Rα was dispensable for Tregs in lymphoid tissues. Mice deficient in thymic stromal lymphopoietin (TSLP), an additional ligand for IL-7Rα, displayed a modest decrease in adipose ST2+ Tregs and a reduced accumulation of adipose eosinophils, accompanied by slightly impaired glucose tolerance. In the VAT, mesothelial cells expressed IL-7, whereas adipose stem cells and folate receptor β-expressing tissue-resident macrophages expressed TSLP. Thus, this study indicates the significance of IL-7Rα signaling in the maintenance of VAT Tregs and glucose homeostasis, revealing a novel role for IL-7 and TSLP in immunometabolism.© The Author(s) 2025. Published by Oxford University Press on behalf of The American Association of Immunologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
Association of 91 Inflammatory Factors and 1400 Metabolites with Sepsis: A Mendelian Randomization Analysis
Hu, Gan, Zhang et al
J Intensive Care Med (2025) 40 (3), 270-283
Abstract: ObjectiveObservational studies suggest links between inflammatory factors, metabolites, and sepsis, yet their causality is uncertain. This study employs Mendelian Randomization (MR) to investigate the causality between these factors and sepsis, aiming to uncover the precise relationship and identify novel treatment approaches.MethodsWe used summary data from genome-wide association studies (GWAS) involving 91 inflammatory factors, 1400 metabolites as exposure, and STREPTO SEPSIS as outcome. Inverse variance weighting (IVW) and MR-Egger were used to evaluate the causal effect between exposure and outcome. Sensitivity analyses were performed using Cochrane's Q test, MR-Egger intercept method, MR-PRESSO method and leave-one-out method.ResultsThymic stromal lymphopoietin levels (TSLP) (OR = 1.269; 95%CI = 1.016-1.585; P = .036) and Interleukin 15 receptor subunit alpha levels (IL-15Rα) (OR = 0.894; 95%CI = 0.801-0.998; P = .046) had a significant causal relationship with sepsis. Forty-four metabolites were associated with sepsis, including Spermidine to choline ratio (OR = 1.447; 95%CI = 1.104-1.977; P = .009), 4-hydroxyhippurate levels (OR = 1.448; 95%CI = 1.117-1.877; P = .005), and Sphingomyelin (d18:1/20:1, d18:2/20:0) levels (OR = 1.371; 95%CI = 1.139-1.651; P < .001). TSLP was associated with 19 metabolites, and IL-15Rα was associated with 30 metabolites.ConclusionsThis study uncovers the causal link between sepsis and two inflammatory factors, TSLP and IL-15Rα, and suggests metabolites' potential in intervention. It also identifies 44 metabolites associated with sepsis, indicating possible biomarkers or therapeutic targets. The findings offer new perspectives on sepsis pathogenesis and could inform future treatment strategies.
Single-cell and chromatin accessibility profiling reveals regulatory programs of pathogenic Th2 cells in allergic asthma
Khan, Alteneder, Reiter et al
Nat Commun (2025) 16 (1), 2565
Abstract: Lung pathogenic T helper type 2 (pTh2) cells are important in mediating allergic asthma, but fundamental questions remain regarding their heterogeneity and epigenetic regulation. Here we investigate immune regulation in allergic asthma by single-cell RNA sequencing in mice challenged with house dust mite, in the presence and absence of histone deacetylase 1 (HDAC1) function. Our analyses indicate two distinct highly proinflammatory subsets of lung pTh2 cells and pinpoint thymic stromal lymphopoietin (TSLP) and Tumour Necrosis Factor Receptor Superfamily (TNFRSF) members as important drivers to generate pTh2 cells in vitro. Using our in vitro model, we uncover how signalling via TSLP and a TNFRSF member shapes chromatin accessibility at the type 2 cytokine gene loci by modulating HDAC1 repressive function. In summary, we have generated insights into pTh2 cell biology and establish an in vitro model for investigating pTh2 cells that proves useful for discovering molecular mechanisms involved in pTh2-mediated allergic asthma.© 2025. The Author(s).
Showing 1-4 of 2521 papers.
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TSLP靶点信息
英文全称:Thymic stromal lymphopoietin
中文全称:胸腺基质淋巴细胞生成素
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:23详情
最高研发阶段:批准上市
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